苦荞总皂苷的提取工艺优化

Technology Optimization for Extracting Total Saponin from Tartary Buckwheat

为了确定热回流法提取苦荞总皂苷的最佳提取工艺条件,采用响应曲面分析方法,研究提取温度、提取时间、液固比、乙醇体积分数及其交互作用对苦荞总皂苷提取效果的影响。用SAS及Design expert软件确定了苦荞总皂苷的最佳提取工艺参数为:乙醇体积分数69.51%,液固比12.37 mL/g,提取温度50.7℃,提取时间63.24 min,总皂苷得率最大值为22.82 mg/g。同一条件下进行验证试验,实际测得的平均得率为(22.54±0.31)mg/g(n=3),试验数据稳定,说明回归模型能较好地预测苦荞中总皂苷的提取得率。

To ascertain the optimal extraction condition of total saponins from tartary buckwheat with thermal re- flux method, the study which using the response surface design, was conducted to investigate the influence of differ- ent extraction temperature, extraction time, liquid - solid ratio, ethanol volume concentration and their cross - ac- tions on saponin yield. Based on the SAS and design expert analysis, the optimum extraction conditions were alcohol concentration of 69.51%, liquid - solid ratio of 12.37 mL/g, extraction temperature of 50.7 ℃, extraction time of 63.24 min. Under the optimized extraction conditions, it can be included that the saponin yield was （22.54 ± 0.31 ） mg/g, and the experimental data was stable, which was close to the estimated value 22.82 mg/g attained by using regression model. This model could be used to predict the experiment results well.