创伤性脑损伤大鼠RSTN基因表达与人参皂苷的干预作用

The RSTN Gene Expression in TBI Rats and Ginsenoside Interference Study

目的:观察创伤性脑损伤大鼠皮层RSTN基因表达的规律及人参皂苷干预对其的影响作用。方法:选取健康SD大鼠48只,随机分为正常对照组(12只)、假手术组(12只)、损伤组(12只)和人参皂苷干预组(12只),制模后按照Longa评分法于术后12 h,24 h,72 h及1周进行神经功能评分,同时取各组大鼠脑皮层组织,采用Western blot法检测RSTN蛋白的表达并采用RT-PCR分析RSTN mRNA的转录。结果:以对照组评分为基准,损伤组在致伤后即刻评分分值升高,到1周一直呈现持续高分值状态。人参皂苷干预组趋势相同(各P均<0.05)。与损伤组比较,人参皂苷干预组在伤后1周的神经功能评分分值出现明显下降,差异有统计学意义(P<0.05);Resistin基因的表达(mRNA和蛋白)在正常对照组和假手术组之间没有显著差异(P>0.05)。以对照组为基准,损伤组在伤后12 h表达量即升高,到伤后1周一直呈持续升高表达(各P均<0.05)。人参皂苷干预组伤后24 h才开始出现表达量的升高,伤后72 h达到峰值,伤后1周时呈现下降(各P均<0.05),且与损伤组比较,人参皂苷干预组各时点RSTN表达的绝对水平均较低(P<0.05)。结论:创伤性脑损伤存在大脑皮质RSTN基因表达的升高,人参皂苷能抑制RSTN基因的高表达,并可能因此发挥对颅脑创伤后继发性脑损伤的保护作用。

Objective：To observe the expression of RSTN gene in traumatic brain injury （TBI） rats and the influence of ginsenoside. Methods ：48 healthy SD rats were randomly divided into normal control group （12）, sham operation group （12） ,TBI group（12） and ginsenoside group（12）. Using homemade free fall Feeney instruments to make heavy brain in- jury model and fill stomach using ginsenoside 5 mg/kg 30 min before making model. All groups＇ rats neurologic evalua- tions were assessed by Longa scals method at 12 h,2g h,72 h and 1 week after operation. The brain cortex samples were gained and RT -PCR and Western blot were used to detect the RSTN gene expression at the same time. Results ：To com- pare with normal group, TBI group showed higher scores on neurologlc evaluation from operation to 1 week. And ginsen- oside group showed lower scores of neurologic evaluation at 1 week. There was no statistically significant difference in RSTN gene expression between normal group and sham operation group（ P 〉 0.05 ）. To compare with normal group, TBI group showed high expression on RSTN at 12 h after trauma and continued to rise until 1 week after trauma（ all P 〈 0. 05）. Ginsenoside group showed high expression on RSTN at 24 h after trauma and peak at 72 h with falling at 1 week （ P 〈 0.05 ）. And compared with TBI group, its absolute level of RSTN gene expression was lower （ P 〈 0. 05 ）. Conclu- s/ons：There are higher RSTN gene expression on brain contex of TBI rats. Ginsenoside could inhibit the expression of RSTN and by the way, may play a protecting role on secondary brain injury in TBI rats.