不同浓度可吸入颗粒物对2型糖尿病大鼠凝血纤溶因子的影响

Study on the blood coagulation FIB rinolytic factors in type 2 diabetic rats exposed to different dose of PM_(2.5)

目的观察不同浓度可吸入颗粒物(PM2.5)对T2DM大鼠凝血纤溶因子的影响。方法采用10%水合氯醛腹腔麻醉,气管内滴注法染毒,24h后处死大鼠,股动脉收集血液,检测凝血酶原时间(PT)、部分凝血活酶时间(APTT)、纤维蛋白原(FIB)、纤溶酶原激活物抑制剂-1(PAI-1)水平。结果不同剂量染毒组PT、PAI-1、FIB与其生理盐水组比较,差异有统计学意义(P<0.05)。滴注相同剂量PM2.5的T2DM大鼠PT、PAI-1、FIB较Wistar大鼠高(P<0.05)。结论不同浓度PM2.5会导致Wistar大鼠凝血纤溶因子的改变,PM2.5对T2DM大鼠凝血纤溶系统的影响更加明显,可能加速糖尿病的进程。

Objective To observe the effect of different concentration of inhalable Particulate Matter（PM2.5）on the blood coagulation FIB rinolytic factors in type 2diabetic rats. Methods The rats were narcotized with 10% chloral hydrate in abdominal and intratracheal instilled with different dose of PM2.5.24 hours later,all rats were sacrificed and their blood was collected from femoral artery.The level of plasma prothrombin time（PT）,activated partial thromboplastin time（APTT）,fibrinogen（FIB）and plasminogen activator inhibitor-1（PAI-1）were detected. Results The levels of PT,PAI-1and FIB in all the PM2.5instilled groups were significant different from saline group（P〈0.05）.The levels of PT,PAI-1and FIB in the same level of PM2.5 instilled T2 DM group were higher than Wister rats（P〈0.05）.Conclusion PM2.5in different concentration can change the blood coagulation FIB rinolytic factors in Wister rats.The effects of PM2.5on blood coagulation and FIB rinolysis system in diabetic rats are more significant which may accelerates the progress of diabetes.