一种用于β_2受体兴奋剂筛查的胶束电动色谱新方法(英文)

A new method for screening of β_2-agonists by micellar electrokinetic chromatography

建立一种快速、简单、有效的胶束电动色谱技术分别检测猪饲料、猪肝、猪肉和猪尿中的5种β2受体兴奋剂.获得优化后的分离缓冲液为15 mmol/L SDS、15 mmol/L硼酸钠、p H=10、以及毛细管温度为25℃.在优化条件下,5种β2受体兴奋剂在18 min内完成分离,在0.1-40μg/m L浓度范围内具有良好的线性关系（R2〉0.999）,其检出限（S/N≥3）在0.05-0.08μg/m L范围之间.西马特罗,莱克多巴胺,克伦特罗具有较好的回收率（n=3）,分别为95.8%,93.1%,95.0%;而沙丁胺醇和特布他林的回收率较低,分别为52.3%和69.4%.被测物的峰面积和迁移时间的相对标准偏差分别小于4.86%和1.25%.利用该方法对5种不同猪饲料、猪肝、猪肉和猪尿样品中的沙丁胺醇,特布他林,西马特罗,莱克多巴胺和克伦特罗进行了筛查。

A method for the determination of five β2-agonists in pig feed,liver,meat,and urine samples by micellar electrokinetic chromatography was developed. The separation and detection conditions were optimized in terms of SDS concentration,running buffer p H and concentration,capillary temperature. Under the optimized conditions（ 15 mmol / L borate,p H = 10,15 mmol / L SDS,and 25 ℃）,all five analytes in the spiking samples were separated and identified within 18 min. Good linearity（ R^2〉0. 999） was obtained for the analytes in the range of 0. 1- 40 μg / m L with the limit of detection（ S / N ≥ 3） in the range of 0. 05- 0. 08 μg / m L. The satisfactory recoveries of cimaterol,ractopamine and clenbuterol were obtained in the range of 93. 1%-95. 8%,whereas the recoveries of salbutamol and terbutaline were at 52. 3% and 69. 4%,respectively. The relative standard deviations of peak area and migration time of the analytes were less than 4. 86% and 1. 25%,respectively. The method was successfully applied to monitor the presence of the selected β2-agonists in pig feed,liver,meat and urine samples.