9种桑黄子实体中两种酚类活性成分的HPLC含量测定

HPLC Determination for Two Kinds of Polyphenols Active Components in Nine Kinds of Phellinus Igniarius Sporocarp

目的通过活性成分的HPLC含量测定,为控制桑黄质量提供依据。方法收集市场上常见的桑黄品种样品,用高效液相色谱法测定hispolon和原儿茶酸含量。色谱柱为UItimate AQ-C18(4.6mm×250mm,5μm);(A)测定hispolon流动相为乙腈-水(38:62),检测波长363nm,(B)测定原儿茶酸流动相为乙腈-0.1%磷酸溶液(12:88),检测波长256nm;柱温为25℃;流速为1m L/min;进样量为10μL。结果 hispolon及原儿茶酸分别在线性范围1.3-0.043mg/m L(r=0.9997),0.4-0.025mg/m L(r=0.9998)线性关系良好。鲍氏针层孔菌、裂蹄针层孔菌、火木针层孔菌、忍冬木层孔菌不同种、不同宿主的9个样品均含有原儿茶酸,含量42.8-96.8μg/g。松木上生长的不同种桑黄,均未检出hispolon,其它样品含有hispolon,含量90.9-223.3μg/g。结论不同桑黄样品均含有原儿茶酸,可作为桑黄的指示成分进行检测。受试样品hispolon含量差异大,表明品种、宿主和生长环境对桑黄成分具有明显影响,影响最大的是宿主,松木上生长的桑黄,均未检出hispolon。监测hispolon含量,对控制桑黄质量,具有重要意义。

Objective To provide the basis for controlling quality of igniarius phellinus , we determined the active constituent contents by HPLC.Method Collecting the common phellinus igniarius varities as the samples , we analyzed the contents of hispolon and protocate -chuic acid using the HPLC method with UItimateAQ -C18 column （4.6mm ×250mm, 5μm） in 25℃ （column temperature）.Hispolon was detected with 38% acetonitrile in water under 363nm （ detecting wavelength ）, and protocatechuic acid was 12% acetonitrile in 0.1%psophate solution at 256nm.The injection volum was 10μL with the flow rate of 1mL/min.Result The liner ranges of hispolon and proto -catechuic acid were 1.3 -0.043mg/mL（r =0.9997）and 0.4 -0.025mg/mL（r =0.9998）, respectively.The contents of protocatechuic acid in the nine kinds of samples （Phellinus baumii, Phellinus linteus, Phellinus igniariusPhellinus lonicerinu ect .） were from 42.8μg/g to 96.8μg/g.Hisplon was not found in live phellinus igniarius on pine tree , but the contents of other verities were from 90.9μg/g to 223.3μg/g.Conclusion Protocatechuic acid was found in all kinds of phellinus igniarius , which could be determined as an indicating ingredient . The content of hispolon in igniarius phellinus was seriously influenced by the varities , parasitifers and growing environment .Determining the content of hispolon has an important implication for the control the quality of phellinus igniarius .