基于彩色CMOS双波长数字全息显微术的细胞相位定量测量

Quantitative Measurement of Cell Phase Using Dual-Wavelength Digital Holographic Microscopy with Color CMOS

提出了一种基于彩色CMOS的双波长数字全息同轴相移显微系统,定量分析人体Jurkat细胞(人外周血白血病T细胞)凋亡过程中的相位变化,实现了在不进行复杂相位解包裹运算的同时对细胞相位的定量测量。利用532 nm和632.8 nm两波长的激光,对人体离体Jurkat细胞凋亡过程中的三个状态进行了彩色全息记录,分别提取出两波长的相位信息,从而重构细胞相位。实验表明,该系统下测得的相位与其他方法测得的相位具有较好的一致性,并且测量量程大,算法简单,结果准确。实验结果将为双波长数字全息术用于细胞相位的研究和应用提供参考。

A dual-wavelength on-axis phase-shifting digital holographic microscopy system based on color CMOS is proposed. Phase variations during the apoptosis process of human jurkat cells （human peripheral blood T cell leukemia） are quantitatively measured and analyzed without complex phase unwrapping algorithm. Using laser with wavelengths of 632.8 nm and 532 nm, color holograms at three states during the apoptosis process ofjurkat cells in vitro are then recorded experimentally. Phase of the cells is reconstructed based on the extracted phase information at each wavelength. The experimental results are consistent with the results obtained by other methods. Moreover, the proposed method described here has wide measurement range and it is simple and accurate. The results indicate that dual-wavelength digital holography is a useful tool for research and application of cell phase.