几种抗生素对蛋白核小球藻的时间毒性微板分析法

Time-dependent Microplate Toxicity Analysis( T-MTA) of Several Antibiotics to Chlorella pyrenoidosa

抗生素在不同的暴露时间可能具有不同的毒性变化规律。本文以蛋白核小球藻(C.pyrenoidosa)为受试生物,96孔微板为暴露实验载体,5种抗生素硫酸安普霉素、氯霉素、双氢链霉素、硫酸新霉素和硫酸链霉素为研究对象,通过在C.pyrenoidosa生长周期内选取6个暴露时间节点(即0、12、24、48、72和96 h),建立了抗生素在不同暴露时间对C.pyrenoidosa生长抑制毒性的微板测试方法(简称T-MTA),并应用T-MTA方法系统测定了5种抗生素对C.pyrenoidosa在不同暴露时间的生长抑制毒性。结果表明,抗生素对C.pyrenoidosa生长抑制毒性具有明显的时间依赖特征,即在开始的时候基本无毒性,而后毒性迅速增加,然后毒性增加速度减慢;不同抗生素的毒性随着暴露时间的延长增加速率不同;同一暴露时间内,5种抗生素对C.pyrenoidosa的毒性大小不同;且毒性顺序随着暴露时间延长而发生变化。

Antibiotics probably have different toxicity features in different exposure times. Selecting Chlorella pyrenoidosa （C. pyrenoidosa） as a test organism, 96-well microplate as exposure experiment carrier and five antibiotics, apramycin sulfate, chloramphenicol, dihydrostreptomycin sesquisulfate, neomycin sulfate and strepomycin sulfate, as test chemicals, a time-dependent microplate toxicity analysis （T-MTA） was developed. Using the T-MTA, the time-dependent toxicities of the five antbiotics on C. pyrenoidosa were determined at six exposure time points such as 0, 12, 24, 48, 72 and 96 h. The results showed that the toxicities of the antibiotics exhibited significant time-dependent features, i.e., they had almost no toxicities on C. pyrenoidosa at first, then their toxicities increased rapidly, and finally increased slowly. However, the antibiotics had different increasing rates for the time-dependent toxicities. The toxicities of five antibiotics were different at the same exposure time. Moreover, the orders of toxicity of the antibiotics at different exposure times were different.