福美双对斑马鱼胚胎Ⅲ型脱碘酶基因表达的影响

Thiram Affects Diodinase 3 Gene Expression in Zebrafish Embryos

福美双是一种二硫代甲氨基甲酸盐类农药,在我国应用较为广泛,但其残留毒性引起广泛重视。试验采用斑马鱼胚胎作为动物模型,探讨了福美双对斑马鱼胚胎的毒性。结果表明福美双导致斑马鱼胚胎孵化率下降,在受精后72 h(72 hpf),对照组的孵化率是100%,1×10-7mol·L-1福美双染毒组的孵化率降至46%,而1×10-6mol·L-1福美双染毒致使孵化率降到0%。III型脱碘酶与斑马鱼的发育和变态息息相关,以上2种浓度的福美双在24 hpf分别增高了III型脱碘酶基因的表达6.71和14.84倍,结果表明福美双具有一定的内分泌搅乱作用,也表明斑马鱼胚胎作为农药安全评价模型的理论可行性。

Thiram is a pesticide of the dithiocarbamate chemical family, and is extensively used in China. Its residual toxicity is a widespread problem and was voiced out by many members of the scientific community. In this study, zebrafish embryos were used as a whole animal model to understand thiram toxicity. Zebrafish era-bryos hatching rate was significantly decreased from 100% in control to 46% in 1 × 10-7 mol.L-1 thiram and e-ven 0% in 1× 10-6 mol. L-1 thiram. At the same time, significant increase in Deiodinase 3, an enzyme crucial in embryonic development and metamorphosis, was observed at 6.71 and 14.84 folds more than control at the two different concentrations at respectively at 24 hpf. These results demonstrated that zebraflsh embryo is a sensitive model to thiram toxicity and can become a useful model for pesticide environmental toxicity assessment.