miR-142-3p对人乳腺上皮细胞MCF-10A增殖及乳蛋白合成的影响

Effect of miR-142-3p on Cell Proliferation of Human Mammary Epithelial Cells MCF-10A and Synthesis of Milk Protein

MicroRNAs(miRNAs)是一类长度约为22nt的非编码的调控性小RNA,在诸多生命活动中发挥重要作用,如参与调控细胞的增殖、分化、凋亡及肿瘤的发生发展。本试验应用脂质体转染技术抑制miR-142-3p在人乳腺上皮细胞的表达。试验采用实时荧光定量PCR、Western blotting、细胞增殖分析等技术,探索miR-142-3p对人乳腺上皮细胞增殖及乳蛋白合成的影响。结果显示,miR-142-3p沉默后,催乳素受体(prolactin receptor,PRLR)蛋白表达增强,同时,相关通路蛋白AKT、mTOR、STAT5、cyclinD1表达量均增加,细胞增殖能力增强。结果表明,在人乳腺上皮细胞中,miR-142-3p的沉默使PRLR蛋白表达量升高,通过调控AKT、mTOR、STAT5、cyclinD1相关通路蛋白而促进乳蛋白质的合成和乳腺上皮细胞的增殖。

MicroRNAs （miRNAs） are a group of small,non-coding RNA molecules about 22 nu- cleotides to regulate a wide variety of important biological processes,including cell proliferation, differentiation,apoptosis as well as the progression of tumors. Liposome transfection was used to detect the expression of miR-142-3p in human mammary epithelial cells,the effects of miR-142-3p on the cell proliferation, apoptosis and milk protein synthesis were detected by Real-time PCR, Western blotting,cell proliferation analysis. The results indicated that after miR-142-3p being si- lenced,prolactin receptor （PRLR） protein was increased,at the same time the expressions of re- lated pathways protein AKT,mTOR,STAT5 and cyclinD1 were increased,the ability of cell pro- liferation was increased. The results suggested that in human mammary epithelial cells, the silence of miR-142-3p could increase the expression of PRLR protein,miR-142-3p could promote the syn- thesis of milk protein and increase the proliferation of mammary epithelial cells by regulating re- lated pathways proteins AKT,mTOR,STAT5 and cyclinD1.