灌服番茄红素对SD大鼠运动后血浆氧化应激的影响

Effects of Intragastric Lycopene on Oxidative Stress of Blood after Exercise SD Rats

试验旨在研究灌服番茄红素对SD大鼠运动后血液抗氧化性能的影响。选取体重为(233.40±6.21)g的健康雄性清洁级SD大鼠32只,随机分为4组,每组8只,对照组饲喂基础日粮,灌胃食用油,试验Ⅰ、Ⅱ、Ⅲ组分别在饲喂基础日粮的基础上灌服10、20、40mg/kg体重番茄红素,预试期10d,正试期42d;每周游泳训练6d,训练6周,游泳11h后采集血液测定氧化应激指标。结果表明,灌服番茄红素后SD大鼠血浆中番茄红素的浓度呈先升高后降低的趋势,在灌服番茄红素后4h时SD大鼠血浆中番茄红素浓度达到峰值;随着灌服番茄红素剂量的增加,SD大鼠运动后血浆谷胱甘肽过氧化物酶活力、总抗氧化酶活力、总抗氧化能力呈升高的趋势;血浆丙二醛的含量随灌服剂量的增加呈降低的趋势。因此,运动后SD大鼠机体抗氧化能力与灌服番茄红素的剂量存在剂量依赖性,随着番茄红素剂量的增加机体抗氧化能力呈升高趋势。

The experiment was conducted to study the effects of intragastric lycopene on oxidative stress blood after exercise SD rats. Thirty two SD rats with similar weight（233.40 g±6.21 g） and good health for SPF were randomized into one control groups and three trial groups with 8 rats per group. The control group was gastric fed with edible oil, the trial Ⅰ , Ⅱ and Ⅲ groups were gastric fed with edible oil intragastric with 10,20,40 mg/（kg · BW） lycopene,respectively. The pre-experiment period lasted for 10 d,and the experiment period lasted for 42 d. Swimming train six days a week and train six weeks,then that blood were collection for determination of oxi- dative stress after swimming train 11 hours SD rats. The results showed that lycopene concentra- tion of plasma was increased at first and decreased subsequently,after 4 h lycopene concentration of plasma in SD rat was peak. With the increase of intragastric lycopene doses,glutathione peroxi- dase,total antioxidant enzyme activity and total antioxidant capacity of plasma showed a trend of rising; malondialdehyde content of plasma showed a trend of decrease with the increase of supple- mental lycopene dose after exercise SD rats. Therefore,the antioxidant capacity of blood after ex- ercise SD rats and doses of intragastric lyeopene were dose dependent, antioxidant capacity of blood after exercise SD rats showed a trend of rise with the increase of lycopene dose.