透骨草水提物与抗菌药对产超广谱β-内酰胺酶大肠杆菌的体外联合抑菌作用研究

Study on the Antibacterial Effects of Queous Extract of Phryma leptostachya L.var.asiatica Hara Combined with Antibiotics invitroagainst ESBLs-producing E.coli

为了探究透骨草水提物与抗菌药联合对产超广谱β-内酰胺酶大肠杆菌的体外抑菌作用。采用二倍微量肉汤稀释法与微量方阵棋盘法来测定各抗菌药与透骨草的最低抑菌浓度(MIC)及二者联合作用后的分级抑菌浓度指数(FICI)。结果显示,透骨草水提物与头孢曲松钠、头孢他啶、头孢噻肟钠、头孢噻呋钠、阿莫西林、阿米卡星、阿奇霉素、磷霉素、黏杆菌素、磺胺间甲氧嘧啶、利福平、环丙沙星、诺氟沙星、左旋氧氟沙星、痢菌净、林可霉素联合作用后的FICI分别为0.75、0.75、1.00、0.75、0.53、0.51、0.75、4.5、4.5、4.5、4.5、2.5。结果表明,透骨草水提物与头孢曲松钠、头孢他啶、头孢噻肟钠、头孢噻呋钠、阿莫西林、阿米卡星、阿奇霉素、磷霉素、黏杆菌素、磺胺间甲氧嘧啶、利福平联合作用时有相加作用;与环丙沙星、诺氟沙星、左旋氧氟沙星、痢菌净、林可霉素联合作用时有颉颃作用。

In order to study the antibacterial effects in vitro of the aqueous extracts of Phryma leptostachya L. var. asiatica Hara combined with antibiotics against ESBLs-producing E. coli, we used broth micro-dilution method and micro-dilution checker-board technique to measure the min- imal inhibitory concentrations （MIC） and the fractional inhibitory concentrations index （FICI） of Phryma leptostachya L. var. asiatica Hara and antibiotics. The results showed that the FICI of Phryma leptostachya L. var. asiatica Hara combined with ceftriaxone sodium, ceftazidime, cefo- taxime sodium, ceftiofur sodium, amoxicillin, amikacin, azithromycin, fosfomycin, colistin, sulfam- onomethoxine, rifampicin, ciprofloxacin, norfloxacin, levofloxacin, mequindox, lincomycin were 0.75,0. 75,1.00,0. 75,0. 53,0. 51,0.75,4.5,4.5,4.5,4.5 and 2.5,respectively. The results of the tests indicated that the aqueous extracts of Phryma leptostachya L. var. asiatica Hara combi- ning with ceftriaxone sodium, ceftazidime, cefotaxime sodium, ceftiofur sodium, amoxicillin, amika- cin, azithromycin, fosfomycin, colistin, sulfamonomethoxine and rifampicin showed additive effect Combining with ciprofloxacin,norfloxacin, levofloxacin, mequindox and lincomycin had the antag- onistic effect.