党参提取物抗菌活性研究

Study on Antibacterial Activity of Extracts of Codonopsis Pilosula

目的:对党参分步提取物的体外抗菌活性进行初步研究。方法:通过测定最小抑菌浓度(MIC)和抑菌圈直径,开展党参的氯仿甲醇提取物、80%乙醇提取物、醇沉液提取物、党参粗多糖提取物对大肠杆菌、金黄色葡萄球菌、链球菌和沙门氏菌的抗菌活性研究。结果:氯仿甲醇提取物、80%乙醇提取物、醇沉液提取物对四种细菌均测到一定的MIC和抑菌圈直径,党参粗多糖提取物对大肠杆菌、金黄色葡萄球菌和链球菌无抑制作用,仅可测到对沙门氏菌的MIC和抑菌圈直径。结论:党参不同的提取物具有明显的抗菌活性差异。

Objective： To study the in vitro antibacterial activity of sequential extracts of Codonopsis pilosula. Methods： By determining the minimum inhibitory concentration（ MIC） and the diameters of inhibition zone,this test studied the antibacterial activity of chloroform methanol extract,80% ethanol extract,80% alcohol layer and Codonopsis pilosula polysaccharide extracts against e.coli,staphylococcus aureus,streptococcus and salmonella. Results： Chloroform methanol extract,80% ethanol extract and 80% alcohol layer have an inhibitory effect on the four types of bacteria,Codonopsis pilosula polysaccharide extracts show no inhibitory effect on e. coli,staphylococcus aureus,and streptococcus,but salmonella. Conclusion： The antibacterial activity of sequential extracts is obviously different.