针刺内关穴对大脑中动脉缺血大鼠血管内皮细胞增殖的调节

Impact of Acupuncture on Positive Regulation of Endothelial Cell Proliferation in Brain Tissue of Rats with Cerebral Ischemia

目的:明确针刺内关穴对大脑中动脉缺血(MCAO)大鼠血管内皮细胞增殖的影响,探讨脑缺血后病理损伤和修复的可能机制。方法:复制大鼠MCAO模型,随机分为4组,模型组、假手术组、非针刺组、针刺组(内关穴3次/s、60s),每组6只模型。4组取材后,通过t-检验结合差异变化倍数(FC)筛选进行差异分析,检测差异表达基因、GO。结果:模型组与假手术组相比,脑缺血病理损伤致基因上调417个,共富集到340个GO生物过程;针刺组与非针刺组相比较,针刺组大鼠神经行为学评分有显著差异,肢体症状较非针刺组减轻(P≤0.05),下调358个基因,共富集到19个GO生物过程。结论:脑缺血损伤导致显著变化的生物过程包括单核细胞聚集、正向调控血管生成、急性时相反应、中性粒细胞趋化性、病毒防御应答、正向调控异型细胞的细胞黏附等,是一个复杂的生物过程;针刺内关穴治疗脑缺血的机制,可能与调控Egr3、Ccl11、Vash2等表达促进大鼠血管内皮细胞增殖有关,通过促进大鼠缺血脑组织脑血管新生,改善脑缺血症状。

Objective： To study the effects of acupuncture on positive regulation of endothelial cell proliferation in brain tissue of rats with cerebral ischemia,and to discuss the mechanism of tissue damage and repair. Methods： MCAO was created by thread embolus,which were randomly divided into four groups with 6 cases in each： model,so,no-acu,acu. The Gene and GO were studied and compared after acupuncture. Results： model VS so： there were 340 different expression GO BP of 417 up-regulated genes; acu VS no-acu ： there were 19 different expression GO BP of 358 down-regulated genes. Conclusion： Acupuncture on Neiguan（ PC6） can promote the recovery of positive regulation of endothelial cell proliferation in brain tissue of rats with cerebral ischemia.