摘要
采用液氨预处理(LAT)法预处理玉米秸秆,通过对预处理条件优化和高效液相色谱(HPLC)法测定酶解液中的单糖含量,考察了温度、含水率、预处理时间等因素对酶解糖化效果的影响,并对整个过程进行了质量平衡分析。实验结果表明,LAT法预处理能显著提高玉米秸秆的酶解转化率;在优化条件下,纤维素酶添加量为15 FPU/(g葡聚糖)时,葡聚糖和木聚糖的72 h酶解率分别为91.8%和82.1%,可达理论转化率的88%,单糖产量是未经预处理原料的2.1倍,每千克干基秸秆原料可生产单糖524.3g。通过与其他预处理方法的对比分析,LAT法预处理后的酶解转化率和单糖产量高于酸、碱、热水、汽爆等预处理方法。
A novel pretreatment called Liquid Ammonia Treatment(LAT) based on AFEX(ammonia fiber expansion) prototype was used to process corn stover in the experiments. The LAT pretreatment conditions such as temperature, moisture content and residence time were optimized, and then the experiments of enzymatic hydrolysis were done. The monosaccharide in enzymatic hydrolyzate was quantified by high performance liquid chromatography(HPLC). The results show that LAT pretreatment can increase the glucan/xylan conversion rate of enzymatic hydrolysis of corn stover significantly. Under optimized conditions, 91.8% glucan and 82.1% xylan conversions were achieved after 72 h enzymatic hydrolysis at 1% glucan loading using 15 FPU/(g of glucan) Cellulase. It is up to 88% of theoretical conversion, and it can produce 524.3 g monosaccharide per kg dry biomass.The LAT-pretreated substrates had a 2-fold higher monosaccharide yield compared with the untreated substrates. The conversion rate is higher than that of acids, alkalis, hot water, and steam explosion pretreatment.
出处
《三明学院学报》
2015年第4期23-29,共7页
Journal of Sanming University
基金
福建省教育厅中青年教师教育科研项目(JA13293)
福建省科技厅重点项目(2012N0023)
福建省科技厅高校产学合作重大项目(2012H6018)
福建省高等学校新世纪优秀人才支持计划(JA13290)
福建省教育厅中青年教师教育科研项目(JB13183)
关键词
玉米秸秆
氨
预处理
酶解
纤维素
乙醇
corn stover
ammonia
pretreatment
enzymatic hydrolysis
cellulose
ethanol