愈伤灵胶囊药效的斑马鱼评价技术探讨

Efficacy Evaluation of Yushangling Capsule by Using Zebrafish as Experimental Animal

以斑马鱼(Danio rerio)为实验动物进行缺氧窒息及游泳能力实验,检测愈伤灵胶囊以及人参皂苷Rb1对斑马鱼的存活、耐受缺氧能力及游泳能力的影响。结果发现,愈伤灵溶液处理1d后,实验鱼窒息时间随药物剂量呈现先增高后降低的趋势,在剂量为0.01mg·L-1时达最大值;实验鱼最大绝对游泳速度呈现类似的变化趋势,不同药物剂量下的实验鱼最大相对游泳速度无显著差异。愈伤灵溶液处理8d后,实验鱼窒息时间随药物剂量呈现降低的趋势,在剂量为0.01mg·L-1时达最大值。人参皂苷Rbl处理8d后,实验鱼窒息时间随药物剂量降低呈现跳跃式陡降。研究结果提示斑马鱼可作为药效评价的模式实验动物,用于中药评价新技术的开发。

This study aimed to obtain the technology to evaluate the efficacy of Yushangling capsule by using zebrafish as the experi- mental animal. The zebrafish was treated by Yushangling and ginsenosides Rbl solution respectively. Then the fish was used to as- say the hypoxia tolerance and swimming performance. The results showed that the suffocation duration increased firstly and then de- creased with the increasing medicine concentration after 24 h of treatment. The maximal suffocation duration was found at 0.01 mg·L^-1. The maximal absolute swimming speed showed similar tendency as hypoxia tolerance, hut the relative speed did not changed significantly. After 8 days of treatment, the suffocation duration was maximal at 0.01 mg·L^-1 and decreased with the in- creasing medicine concentration. The suffocation duration of the fish treated by ginsenosides Rbi showed a shock drop with the in- creasing concentration. It suggests that the zebrafish could be used to judge the efficacy of medicines, which could contribute to the new evaluating technology of Chinese medicines in further study.