通络刮痧法对肝郁血瘀型乳腺增生大鼠乳房微循环及血管生成的影响

Influence of Tongluo Guasha therapy on microcirculation and angiogenesis in breasts of hyperplasia of mammaryglands rats with syndrome of stagnation of liver qi and blood stasis

目的:探索通络刮痧法对肝郁血瘀型乳腺增生大鼠乳房微循环及血管生成的影响及作用机制。方法:将60只SD雌鼠随机分为空白组、模型组、早预防组、预防组、治疗组、晚治疗组。各预防组和治疗组采用通络刮痧治疗。观察造模前后及治疗后各组动物行为学改变、乳头大小的变化、血液流变学及血管内皮生长因子(VEGF)、碱性成纤维生长因子(b FGF)的表达,并观察乳房病理形态学改变。结果:与空白组相比,其它各组大鼠的乳头高度、直径增加明显(P<0.05),血液流变学各项指标明显上升(P<0.05),乳腺组织中VEGF、b FGF的表达明显增强(P<0.05);早预防组、预防组、治疗组和晚治疗组与模型组相比,各项指标都有所下降(P<0.05)。结论:通络刮痧可能通过降低血液黏稠度、抑制乳腺血管生成来预防和治疗乳腺增生。

Objective： To explore the effects and mechanism of Tongluo Guasha therapy on microcirculation and angiogenesis in breasts of hyperplasia of mammaryglands rats with syndrome of stagnation of liver qi and blood stasis. Methods： Sixty SD rats were randomly divided into a control group, model group, early prevention group, prevention group, treatment group and delayed treatment group. Each prevention and treatment group was treated with Tongluo Guasha therapy. The animal behavior changes, the size of breast, the expression of VEGF and b FGF, hemorheology changes, and the pathomorphology changes of breast were observed before and after the treatment. Results： Compared with the control group, the height and diameter of breast nipple, parameters of hemorheology and expression of VEGF and b FGF in breasts of rats in the other groups were increased significantly（P〈0.05）. Compared with model group, indicators of rats in early prevention group, prevention group, treatment group and delayed treatment group were decreased significantly（P〈0.05）. Conclusion： Tongluo Guasha therapy could reduce blood viscosity and inhibit angiogenesis in breast, which has positive significance in prevention and treatment of hyperplasia of mammary glands.