Cascade Enzymatic Hydrolysis Coupling with Ultrafine Grinding Pretreatment for Sugarcane Bagasse Saccharification

为甘蔗甘蔗渣 saccharification 的 biorefinery 过程通常要求纤维素的重要可接近性。我们报导了串联绗维素酶的一个新奇方法为甘蔗甘蔗渣 saccharification 结合极其细小的磨擦预告的处理的酶的水解作用。包括的三个酶的水解作用模式挑选绗维素酶酶的水解作用，混合绗维素酶酶的水解作用，和串联绗维素酶酶的水解作用被比较。功能的组和在串联绗维素酶期间，酶的水解作用也是的甘蔗渣的表面形态学上的变化分别地由英尺红外和 SEM 检验了。结果显示出酶的水解作用是的那串联提高甘蔗甘蔗渣 saccharification 的最有效的方法。与90.1%葡萄糖选择减少糖产量中的超过65%个在 50 摯椠?桷捩?被完成?眠獡甠敳?獡愠獳獩楴杮映畬?湡??慬杲?整灭牥瑡牵?牧摡敩瑮???鰠??釦?鳛???鴿鮘???微鴿钤??颞酋?鱎?隝????龜?徬?疞?螮趬鐿?????????吗????？？

The biorefinery process for sugarcane bagasse saccharification generally requires signifcant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrol- ysis coupling with ultrafine grinding pretreatment for sugarcane bagasse saccharification. Three enzymatic hydrolysis modes including single cellulase enzymatic hydrolysis, mixed cellulase enzymatic hydrolysis, and cascade cellulase enzymatic hydrolysis were compared. The changes on the functional group and surface morphology of bagasse during cascade cellulase enzymatic hydrolysis were also examined by FT-IR and SEM respectively. The results showed that cascade enzymatic hydrolysis was the most efficient way to enhance the sugarcane bagasse saccharification. More than 65% of reducing sugar yield with 90.1% of glucose selectivity was achieved at 50 ℃, pH=4.8 for 72 h （1200 r/min） with cellulase I of 7.5 FPU/g substrate and cellulase II of 5 FPU/g substrate.