注射诱导次数对黄粉虫抗菌肽含量及体外抑菌效果的影响

Effect of the times of induction by injecting on the quantity of antimicrobial peptide extraction and the bacteriostatic activity extracorporeal

目的:本研究旨在研究不同注射诱导次数对黄粉虫抗菌肽含量及粗提液体外抑菌活性的影响。方法:将200头四龄黄粉虫分成四组,每组50头。用灭活大肠杆菌菌悬液按1μL/头剂量注射到四龄黄粉虫体内,第1组为对照组,不予注射诱导,第二至第四组为诱导组,分别注射诱导1、2、3次。每两次注射诱导间隔为24 h,注射诱导24 h后研磨破碎活虫虫体加入一定的提取液提取抗菌肽。将四组抗菌肽粗提液用微量肉汤稀释法测定MIC值以比较各组体外抑菌活性。结果:注射诱导次数对黄粉虫抗菌肽含量与体外抑菌效果均有极显著性影响(P<0.01)。

The main objective of this study is to find out the quantity of antimicrobial peptide extraction base on the different number of injection stimulate and explore the bacteriostatic activity of antibacterial peptides crude extract. [Methodology]200 four age Tenebrio molitors divided into four groups, 50 heads in each group. The inactivation of E.coli was injected into the suspension of Tcnebrio molitor , 1μl of each injection.The first group was the control group, without injection induced; The second to the fourth groups are for the induced group, The second, group was induced once, the third twice and the fourth 3 times. The injection interval of each two injections was 24hours, after 24 hours, grinding live bugs extraction of antimicrobial peptides with extraction. To compa We have observed the quantity of antimicrobial peptide extraction base on the different number of injection stimulated, and e re the antibacterial activity of the groups in vitro, the MIC of four groups of antimicrobial peptides crude extract was determined by two times dilution method. [Conclusion]xplore the bacteriostatic activity of antibacterial peptides crude extract. The Influenced by the difference of injection induce; both the antimicrobial peptide extraction content and antibacterial peptides＂ bacteriostatic activity is present significant variation.each group. The inactivation of E.coli was injected into the suspension of Tenebrio molitor , 1 Dxl of each injection.The first group was the control group, without injection induced; The second to the fourth groups are for the induced group, The second group was induced once, the third twice and the fourth 3 times. The injection interval of each two injections was 24hours, after 24 hours, grinding live bugs extraction of antimicrobial peptides with extraction. To compa We have observed the quantity of antimicrobial peptide extraction base on the different number of injection stimulated, and e re the antibacterial activity of the groups in vitro, the MIC of four groups of antimicrobial peptides crude extract was determined by two times dilution method. [Conclusion ]xplore the bacteriostatic activity of antibacterial peptides crude extract. The Influenced by the difference of injection induce; both the antimicrobial peptide extraction content and antibacterial peptides＇ bacteriostatic activity is present significant variation.