香菜提取液的抗氧化活性初步研究

Antioxidant Activity of Coriandrum sativum Extract

采用煮制法提取香菜中的有效成分,研究其体外抗氧化活性。运用硝酸铝显色法,检测总黄酮含量,并测定香菜提取液的总抗氧化能力以及其对羟自由基的清除能力。结果表明,芦丁在0.01~0.1 mg/mL（R2=0.999 1）质量浓度范围内,吸光度与浓度之间呈良好线性关系,香菜提取液的黄酮含量为1.73 mg/g鲜重;香菜具有显著的抗氧化活性,且呈剂量依赖性,总抗氧化能力为44.19 mg VC/g鲜重,其清除羟自由基的能力IC50=67.11 mg/mL。由此说明了香菜具有丰富的黄酮类化合物,且具有良好的体外抗氧化活性。

Aim to extract the Coriandrum sativum by cooking, then evaluate the antioxidant activity in vitro. The content of total flavonoids was measured by AI（NO3）3 assay. It used the Coriandrum sativum extract to determine the total antioxidant capacity and the hydroxyl radical scavenging （.OH）. The results showed that the linear range of rutin was 0.01-0.1 mg/mL （R2=0.999 1）. The content of total flavonoids is 1.73 mg/g. The Coriandrum sativum extract exhibited antioxidant activity in a dose-dependent manner. The total antioxidant capacity is 44.19 mg VC/g. The IC50 of the hydroxyl radical scavenging （.OH） is 67.11 mg/mL. Moreover, the Coriandrum sativum is rich in flavonoids and possesses high antioxidant activity.