基于Biolog-FF技术的金霉素降解真菌的碳代谢特征研究

On carbon metabolism of fungi in chlortetracycline degradation based on Biolog-FF system

【目的】利用Biolog-FF技术对4种不同金霉素降解真菌代谢95种碳源特征进行测定分析。【方法】测定不同时段4种真菌对95种碳源代谢的吸光值,并将4种真菌分别接种到金霉素药渣固废中,测定不同发酵时间药渣中的总有机碳含量。【结果】4种真菌利用碳源种类和活性差异较大,桔青霉LJ318、哈茨木霉LJ245、小刺青霉LJ236、草酸青霉LJ302能够利用碳源数量依次为41、39、15和14种;菌株LJ245和LJ318利用碳源的平均活性显著高于菌株LJ236和LJ302;4种真菌能够较好利用的碳源类型为糖类、氨基酸类、聚合物类等物质。【结论】菌株LJ245和LJ318代谢药渣中的碳源明显快于菌株LJ236和LJ302,这与Biolog方法测定结果趋势一致。Biolog-FF技术是一种快速测定真菌单菌落碳代谢特征的有效方法。研究为探讨真菌碳代谢特征与生物降解环境残留金霉素提供了科学依据。

[Objective] Biolog-FF system with 95 kinds of carbon source was used to exploit the metabolism feature of four fungi in chlortetracycline degradation. [Methods[ The changes of absorbance values of the four strains in 95 kinds of carbon sources were determined in different time. The total organic carbon （TOC） contents were measured after the four strains were inoculated into the chlortetracycline solid waste in different time. [Results] The four strains digested different carbon sources, and the activities of them were quite distinct. 41 kinds, 39 kinds, 15 kinds and 14 kinds of carbon were used by Penicillium citrinum LJ318, Trichoderma harzianum LJ245, Penicillium spinulosum LJ236 and Penicillium oxalicum L J302, respectively. The average activity digesting carbon of LJ245 and LJ318 were significantly higher than that of the strain LJ236 and LJ302. All the four strains could utilize sugars, amino acids, polymers and so on. [Conelusion] Carbon metabolism of LJ245 and LJ318 in residue were significantly faster than LJ236 and L J302. This show the same results as what the Biolog system drew out. Biolog technology is an effect method in the study of carbon metabolic characteristics of fungi. This study would build a scientific foundation for investigating the characteristics of fungal carbon metabolism, and for the bio-degradation of chlortetracycline in environment.