江苏水稻品种稻瘟病主效抗性基因鉴定及应用评价

Identification and Evaluation on Blast Resistance of Rice Varieties in Jiangsu

以28个抗稻瘟病单基因系和740个水稻品种为材料,在江苏省连云港市的水稻种植区进行稻瘟病抗性的田间鉴定。结果表明,28个单基因系中藤板5号(Pii)、砦1号(Pizt)、F-80-1(Pik)、F-124-1(Pita)、F-98-1(Pikm)、F-128-1(Pita2)、C101LACC[Pi1(t)]、C101A51[Pi2(t)]和C104PKT[Pi3(t)]等表现较高的抗性,供试材料中高抗、抗、中抗、中感、感和高感的品种数分别是97、230、200、93、48和55个。从中选出Pii、Pikh、Pi1、Pi2、Pita、Pik、Pikm、Pikp和Pib,并增加了广谱高抗基因Pi9,对其中的195个高抗、高感和重要品种进行抗性基因功能标记分析,结果表明,含有Pii、Pikh、Pita、Pib、Pi2和Pik的品种数分别有28、40、52、110、11和2个,未检测到含有Pi1、Pikm、Pikp和Pi9的品种。除Pib外,其他3个检测到的基因基本都存在于抗性或高抗品种中。

Twenty-eight monogenic lines and 740 varieties of rice were examined for their resistances to blast in the fields in Lianyungang,Jiangsu.Among the 28 monogenic samples,Fujisaka 5 （Pii ）,Taride 1 （Pizt ）,F-80-1 （Pik）,F-124-1 （Pita ）,F-98-1 （Pikm ）,F-128-1 （Pita 2 ）,C101LACC [Pi1 （t ）],C101A51 [Pi2 （t ）]and C104PKT [Pi3 （t）]exhibited high resistances to blast.Nighty-seven of the 740 cultivars were found to be highly resistant,230 resistant, 200 moderate resistant, 93 moderately susceptible, 48 susceptible, and 55 highly susceptible to the blast.To evaluate the genetic effect of these resistant genes,195 samples with high resistance or susceptibility to the blast were selected.The important cultivars were analyzed on their molecular markers with 10 functional ones for the resistant genes.The result showed that the locus of Piiwas found in 28 of the materials, Pikhin 40,Pitain 52,Pib in 110,Pi2 in 11,and Pik in 2. On the other hand,no locus of Pi1,Pikm,Pikp orPi9was detected in these varieties.