基于RNA干扰技术探讨消岩汤对肺腺癌A549细胞凋亡的影响

Investigation on influence of Xiaoyan Decoction on lung adenocarcinoma cancer cell A549 apoptosis based on si RNA interference

目的分析消岩汤干预下sh-survivin对人肺腺癌A549细胞增殖及凋亡的影响。方法选择人肺腺癌A549细胞,采用中药复方进行干预及sh RNA进行转染,将转染后的细胞分组,通过MTT法观察消岩汤对A549细胞生长的影响,免疫组化法检测survivin蛋白的表达,流式细胞仪检测细胞凋亡率。结果消岩汤能有效地抑制人肺腺癌A549的细胞增殖,运用消岩汤作用细胞24 h后,侵袭的细胞数明显减少,该方作用48 h后可以诱导肿瘤细胞凋亡;消岩汤中剂量组与转染sh-survivin组抑制率相当,不同剂量的消岩汤均可协同sh-survivin使肺癌细胞凋亡率增加。结论消岩汤可以通过干预肿瘤细胞凋亡抑制蛋白的表达而有效抑制肿瘤的发展。

Objective To analyze the effect of Xiaoyan Decoction intervened sh-survivin human lung adenocarcinoma A549 cell proliferation and apoptosis. Methods Selecting the human lung adenocarcinoma A549 cells, using the intervence of compound Chinese materia medica and sh RNA transfection, after transfection cells wre grouped, the effect of Xiaoyan Decoction on the proliferation of human lung adenocarcinoma A549 cell was determined by MTT method, the Survivin protein expression was detected by immunohistochemical method, and the cell apoptosis rate was detected by flow cytometry instrument. Results Xiaoyan Decoction could effectively inhibit the cell proliferation of human lung adenocarcinoma A549; Using Xiaoyan Decoction on cells after 24 h, the number of cells was significantly decreased, the tumor cell apoptosis was induced after 48 h of the administration of Xiaoyan Decoction. The inhibitory rate of Xiaoyan Decoction in treatment dose group and transfection group was equal, the Xiaoyan Decoction at different dosage can be coordinated with sh-survivin to increase rate of lung cancer cell apoptosis. Conclusion Xiaoyan Decoction can inhibit the tumor development by intervening tumor cell apoptosis and depressing the protein expression..