乳腺癌转移淋巴结T细胞克隆性分析

Analysis of clonal espression of T cells in metastatic lymph node of patients with breast cancer

目的分析乳腺癌患者转移淋巴结中TCR BV亚家族T细胞的克隆性分布特点。方法选取2例反应性增生淋巴结及10例乳腺癌转移淋巴结标本,应用RT-PCR方法扩增T细胞24个TCRβ链可变区（BV）亚家族的互补决定区3（CDR3）基因,经基因扫描确定T细胞克隆性。结果乳腺癌转移淋巴结中T细胞存在克隆性增生,增生形式包括单、寡克隆及多克隆。不同患者增生T细胞克隆中均存在TCR谱型偏移,仅表达2~5个BV亚家族。结论乳腺癌转移淋巴结内克隆性T细胞TCR BV亚家族存在选择性取用特点,可能与机体对乳腺癌相关抗原的特异性免疫反应有关。

Objective To investigate the distribution and clonality of TCR BV subfamilies T cells in patients with breast cancer. Methods The CDR3 region genes of 24 TCR BV subfamilies were amplified by RT-PCR in T cells from 2 reactive hyperplasia lymph nodes and 10 patients from lymph node metastasis,and the CDR3 size lengths of TCR BV were analyzed by Gene Scan technique for detection of the CDR3 size,to evaluate clonality of T cells. Results TCR BV presented specific repertoire skewing in 5 cases in metastatic lymph node,and only 2 ~ 5 TCR BV subfamilies T cells were identified. Clonal expanded T cells,including monoclonal、oligoclonal and multiclonal patterns. Conclusion There were T cells clonal expansion and selected usage of TCR BV subfamilies in metastatic lymph node of patients with breast cancer,it may be related to breast cancer associated antigen.