荧光偏振检测技术在布鲁杆菌病诊断中的对比实验分析

A comparison of fluorescence polarization assay with four classical serological methods in diagnosis of human brucellosis

目的 分析荧光偏振检测方法对布鲁杆菌病（简称布病）的诊断价值.方法 2013年8月至2014年4月,在黑龙江省农垦总局总医院选择布病患者240例作为病例组,正常体检人员287例作为对照组,抽取空腹静脉血4 ml,分离血清.采用荧光偏振检测方法（FPA）和4项传统血清学检测方法[包括虎红试验（RBPT）、试管凝集试验（SAT）、抗人免疫球蛋白试验（Coomb＇s试验）和半胱氨酸试验]进行检测,比较FPA与4项传统血清学检测方法的一致性、灵敏度和特异度.结果 FPA与4项传统血清学检测方法均有较好的一致性（Kappa值均≥0.75）;FPA检测方法的敏感度（89.17％）高于RBPT （87.08％）、SAT （85.00％）、Coomb＇s试验（74.17％）和半胱氨酸试验（75.83％）.FPA检测方法的特异度（94.08％）高于RBPT（89.90％）、Coomb＇s（81.53％）,但低于SAT（98.95％）、半胱氨酸凝集试验（99.30％）.结论 FPA可以作为人间布病的实验室筛查诊断方法,值得广泛推广使用.

Objective To analyze the diagnostic value of fluorescence polarization assay （FPA） for human brucellosis.Methods From April 2013 to August 2014,240 positive sera of brucella patients were collected as case group and 287 normal sera as control group in Heilongjiang Agricultural Reclamation Bureau General Hospital.Four milliliters of venous blood were withdrawn for separation of serum.Fluorescence polarization assay was used and the consistency,sensitivity and specificity were compared with those of four classical serological methods,including rose bengal plate agglutination test （RBPT）,standard tube agglutination test （SAT）,Coomb＇s test and cysteine test.Results The results of FPA were complied with those of the four conventional serological methods,Kappa ≥ 0.75.The sensitivity of FPA （89.17%） was higher than that of RBPT （87.08%）,SAT （85.00%）,Coomb＇s （74.17%） and cysteine test （75.83%）.The specificity of FPA （94.08%） was higher than that of RBPT （89.90%） and Coomb＇s （81.53%）,but lower than that of SAT （98.95%） and cysteine test （99.30%）.Conclusion FPA can be used as a laboratory diagnostic method for human brucellosis,which is worthy to be widely used.