小鼠盲肠系膜三角原位种植结肠癌模型的构建与评价

Establishment and evaluation of a novel mouse model of orthotopic colon cancer in the mesenteric triangle of the cecum

目的：探讨经盲肠系膜三角注射肿瘤细胞悬液制备小鼠结肠癌原位种植模型的可行性。方法手术暴露小鼠盲肠，将小鼠结肠腺癌CT26． WT细胞接种于盲肠系膜三角，建立小鼠结肠癌原位种植模型。结果20只BAL B／c小鼠经盲肠系膜三角注射肿瘤细胞悬液制备小鼠结肠癌原位种植模型的成瘤率为100％，且不易发生梗阻，但可发生肝、脾、横膈、肠系膜淋巴结等多处转移。结论经盲肠系膜三角注射肿瘤细胞悬液制备小鼠结肠癌原位种植模型手术时间短，不易损伤结肠壁，对小鼠的创伤小，手术风险小；手术操作简单，易于掌握。

Objective To explore the feasibility of preparation of a mouse model of orthotopic colon cancer by injecting tumor cell suspension into mesenteric triangle of the cecum. Methods Twenty SPF 8-week old BALB/c mice （ male ∶ female=1 ∶ 1） were used in this study. The mouse caecum was exposed by laparostomy, and suspension of mouse colon adenocarcinoma CT26.WT cells was injected into the mesenteric triangle of cecum for preparation of a mouse model oforthotopic colon cancer. Results Mouse orthotopic colon cancer was developed by injection of tumor cell suspension into mesenteric triangle of the cecum showing a successful rate of 100%, without intestinal obstruction, and the liver, spleen, diaphragm and mesenteric lymph nodes metastasis rates were high in all the 20 experimental mice. Conclusions The establishment of mouse models of orthotopic colon cancer by injection of tumor cell suspension into the mesenteric triangle is a simple, rapid, and easy to master procedure, causing less damage to the colon wall, safe and with less trauma to the mice. This method may provide an ideal mouse model of orthotopic colon cancer for the study of pathogenesis as well as liver metastasis mechanisms of colon cancer.