骨桥蛋白基因剪切体在胃癌组织中的表达及意义

Expression of osteopontin splice variant and its clinical significance in gastric cancer

目的：研究胃癌组织中骨桥蛋白（ OPN）基因剪切体OPN-A、OPN-B和OPN-C mRNA的表达，及其在胃癌发展和转移过程中的作用。方法采用实时荧光定量PCR法检测66例胃癌组织和癌旁正常组织中OPN-A mRNA、OPN-B mRNA和OPN-C mRNA的表达差异，分析OPN-A mRNA、OPN-B mRNA和OPN-C mRNA的表达与胃癌患者临床病理特征及预后的关系。结果 OPN-A mRNA和OPN-B mRNA在胃癌组织中的表达量分别为癌旁正常组织的1．06和1．27倍，差异无统计学意义（均P＞0．05）；而OPN-C mRNA在胃癌组织中的表达量为癌旁正常组织的3．21倍，差异有统计学意义（P＝0．001）。 OPN-A mRNA和OPN-B mRNA在胃癌组织中的表达与胃癌的浸润深度、肿瘤直径、有无远处转移、淋巴结转移情况以及分化程度均无关（均P＞0．05）；而OPN-C mRNA的表达与肿瘤的浸润深度、肿瘤直径、有无远处转移、淋巴结转移情况有关（均P＜0．05），仅与肿瘤的分化程度无关（P＞0．05）。 OPN-C mRNA高表达组患者的中位生存时间为14个月，明显低于低表达组患者（29个月， P＝0．03）。结论 OPN-C可能促进胃癌的发展和转移，而OPN-A和OPN-B的表达与胃癌的发展和转移无明显关系。 OPN-C mRNA表达水平可作为评估胃癌患者预后的分子指标。

Objective To investigate the expression of osteopontin （ OPN） splice variant mRNA, including the three isoforms OPN-A, OPN-B, and OPN-C, to explore its correlation with clinicopathologic features in gastric cancer, and to elucidate their role in tumor invasion and distant metastasis of gastric cancer. Methods The expression of OPN-A, OPN-B and OPN-C mRNA were detected by real-time reverse transcriptase-polymerase chain reaction in 66 gastric cancer tissues. The relationship between the expression of OPN-A, OPN-B and OPN-C mRNA and clinicopathologic features of gastric cancer was analyzed. Results The expression of OPN-C mRNA in the gastric cancer tissue was 3. 21-fold higher than that in peritumoral mucosal tissue, showing a significant difference between them （P〈0.001）. OPN-C mRNA expression was correlated with the depth of tumor invasion, tumor diameter, lymph node meatastasis, distant meatastasis and had no correlation with differentiation grades. The low expression of OPN-C mRNA was correlated with long survival benefit （ P=0.03） . The expression of OPN-A and OPN-B mRNA had no significant relationship with clinicopathologic features of gastric cancer. Conclusions One of the isoform of osteopontin （ OPN） OPN-C mRNA is overexpressed in gastric cancer. The overexpression of OPN-C mRNA may reflect the progression and is associated with the prognosis of gastric cancer. OPN-C mRNA may have value as a prognostic biomarker in gastric cancer. However, the expression of OPN-A and OPN-B are not correlated with the progression and metastasis of gastric cancer.