逆灸对不同运动强度小鼠心肌细胞的保护作用

Protective Effect of Preventative Moxibustion on Myocardial Cells in Mice Experiencing Moderate-intensity and Exhausted Exercise

目的:探讨逆灸对不同运动强度小鼠心肌细胞凋亡损伤的影响。方法:将60只昆明小鼠随机分为空白对照组、空白逆灸组、中等运动对照组、中等运动逆灸组、力竭运动对照组、力竭运动逆灸组,每组10只。采用不同运动强度对小鼠进行训练。各逆灸组取"足三里""关元"穴进行艾灸治疗,每穴每日灸5壮,每日1次,每周休息1d,共治疗3周。采用TUNEL法检测各组心肌细胞凋亡率,电镜观察各组心肌亚细胞结构破坏程度。结果:中等运动对照组与空白对照组相比,心肌细胞凋亡率升高(P<0.05);中等运动逆灸组与中等运动对照组相比心肌细胞凋亡率下降(P<0.05);力竭运动对照组与中等运动对照组相比,心肌细胞凋亡率升高(P<0.05);力竭运动逆灸组与力竭运动对照组相比,心肌细胞凋亡率明显下降(P<0.05)。电镜观察显示,中等运动逆灸组比中等运动对照组心肌细胞亚细胞结构破坏程度轻,力竭运动逆灸组比力竭运动对照组心肌细胞亚细胞结构破坏程度轻。结论:不同运动强度的运动训练均可引起心肌细胞发生凋亡损伤,且伴有心肌组织损伤,随着运动强度加大,心肌细胞发生凋亡更加明显;艾灸可减轻高强度运动对小鼠心肌细胞造成的损伤,减少细胞的凋亡。

Objective To observe the effect of preventative moxibustion on cardiomyocytes in mice experiencing different intensities of exercise.Methods Kunming mice were randomly divided into blank control（n=10）,blank-moxibustion（n=10）,moderate-intensity exercise（MIE）model（n=8）,MIE-moxibustion（n=9）,exhausted-exercise model（n=8）,and exhausted-exercise moxibustion（n= 8）groups.Preventative moxibustion was applied to ＂Zusanli＂（ST 36）and ＂Guanyuan＂（CV 4）for 5min,once daily（except Saturdays or Sundays）for 3weeks.The apoptotic cardiomyocytes were detected by light microscope after terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling（TUNEL staining）,and the ultrastructure of cardiomyocytes was observed by using transmission electron microscope.Results Compared with the blank control group,the rates of the apoptotic cardiomyocytes were considerably increased in both MIE and exhausted-exercise model groups（P〈0.05）.Comparison between the MIE model and MIE-moxibustion groups and between the exhausted-exercise model and exhausted-exercise moxibustion groups showed that the apoptotic rates of cardiomyocytes were significantly lower in the two moxibustion groups than in the two model groups（P〈0.05）.In mice experiencing preventative moxibustion,the injured state of the cardiomyocytes including dissolved myofilaments of myocardial fibers,disorder of arrangement and increased interspace of myofilaments,and decreased number of partial myocardial bundles,and the increase of matrix electron density was relatively milder in comparison with their individual exercise model groups.Conclusion Preventative moxibustion may reduce MIE and exhausted exercise induced apoptosis of cardiomyocytes in mice and lessen myocardial injury.