食管鳞癌组织中HIF-1α和VEGF-C的表达及意义

Expression and significance of hypoxia-inducible factor-lα and vascular endothelial growth factor-C in esophageal squamous cell carcinoma tissues

目的研究食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中缺氧诱导因子(hypoxia inducible factor-1α,HIF-1α)、血管内皮生长因子-C(vascular endothelial growth factor,VEGF-C)的表达及其与临床病理特征的关系。方法采用免疫组织化学(SP)法检测116例ESCC及20例癌旁组织组织中HIF-1α、VEGF-C蛋白的表达,并分析其与临床病理特征的关系。结果 HIF-1α与VEGF-C在ESCC组织中的阳性表达率均高于癌旁组织(P<0.05)。HIF-1α蛋白的阳性表达与T分期相关(P<0.05)。VEGF-C与分化程度、淋巴结转移相关(P<0.05)。HIF-1α与VEGF-C蛋白表达之间存在着显著的正相关关系(r=0.331,P=0.000)。结论 HIF-1α、VEGF-C蛋白在ESCC组织中高表达,可能在ESCC发生发展中有重要作用。VEGF-C表达与淋巴结转移有关,可作为预测ESCC预后的重要指标。

Objective To investigate the expression of hypoxia -inducible factor -1α（HIF -1α）and vascular endothelial growth factor -c（VEGF -C）in esophageal squamous cell carcinoma（ESCC）tissues and the correlation with the clinicopatholog-ical features.Methods The expression of HIF -1αand VEGF -C in 106 cases of esophageal squamous cell carcinoma tissues and 20 cases of adjacent esophageal mucosa were detected by immunohistochemical staining,and the correlation with clinicopath-ological features were analyzed.Results The positive expression rates of HIF -1αand VEGF -C were higher than adjacent e-sophageal mucosa（P ＜0.05 ）.The expression of HIF -1αwas correlated with T stage （P ＜0.05 ）,and the expression of VEGF -C was correlated with differentiation and lymph metastasis （P ＜0.05）.There was a significantly positive correlation be-tween HIF -1αand VEGF -C protein expression（r =0.331,P =0.000）.Conclusion The expression levels of HIF -1αand VEGF -C were higher,and they may play important roles in the development and progression of ESCC.The expression of VEGF -C was correlated with lymph metastasis,and it may be used as an important index to predict the prognosis of ESCC.