结核感染干扰素释放试验中的QFT-G试验在肺结核诊断中的应用评价

Clinical Application of IFN-γ Release Assays for Rapid Diagnosis of Active Tuberculosis

目的探讨结核感染干扰素释放试验中的QFT-G试验在肺结核诊断中的应用评价。方法采用QFT-G检测外周血结核特异性γ-干扰素的含量,同时与FQ-PCR检测痰中结核分枝杆菌核酸结核菌素、纯化蛋白质衍生物(PPD)皮试进行平行比较分析。结果 QFT-G、FQ-PCR和PPD三种方法的敏感度分别为:91.4%、84.2%、78.9%,特异性分别为86.9%、80.8%和54.8%,阳性预测值分别为91.0%、92.2%和71.1%,阴性预测值分别为66.2%、75.4%和46.3%,准确性分别为87.8%、85.0%和59.6%。结论 QFT-G较FQ-PCR和PPD有较高的敏感度、特异性和准确性,是一种诊断肺结核的有价值的检测方法。

Objective To investigate the clinical application of IFN-γ release test in diagnosis of pulmonary tuberculosis. Methods The concentration of mycobacterium tuberculosis specific IFN-γ in peripheral blood was detected by QFF-G array. The M. tuberculosis DNA loads were examined by FQ-PCR. The results were compared PPD test. Results The sensitivity of QFF-G array, FQ-PCR and PPD was 91.4% ,84.2% and 78.9% ; the specificity was 86.9% ,80.8% and 54.8% ; the positive predictive value was 91.0% ,92.2% and 71.1% ; the negative predictive value was 66.2% ,75.4% and 46.3%, and the accuracy was 87.8%, 85.0% and 59.6%. Conclusion Compared with FQ-PCR and PPD, QFT-G array had more level of sensitivity, specificity and accuracy, and it may be used as a valued index to evaluate tuberculosis infection.