CD166及转录因子MALAT1基因在非小细胞肺癌的表达分析

Expression of CD166 and Transcription Factor MALAT1 Gene in Non-small Cell Lung Cancer

目的分析肿瘤干细胞标志物CD166及肺腺癌转移相关转录子1(metastasis-associated lung adenocarcinoma transcript1,MALAT1)于非小细胞肺癌(non-small cell lung cancer,NSCLC)发生、发展中的作用,以期为临床提供参考。方法从2013年9月至2014年9月我院呼吸内科实施肺癌根治切除术患者中选取100例作为研究对象,经病理诊断均为NSCLC。选取新鲜NSCLC组织作为观察组,癌旁组织为对照组,两组均实施免疫组化法检测CD166表达,采用RT-PCR对MALAT1中mRNA表达情况进行检测。结果观察组CD166阳性表达率显著高于对照组。观察组MALAT1表达(87.61±9.25)显著高于对照组(40.54±5.83),差异均有统计学意义,P<0.05。不同性别、年龄患者间的MALAT1表达差异均无统计学意义,与病理分级相关,其差异均有统计学意义;不同性别、年龄患者间的CD166表达差异均无统计学意义,P>0.05,与病理分级更加相关,差异均有统计学意义,P<0.05。结论在NSCLC发生、发展过程中CD166及转录因子MALAT1发挥了重要作用,对其进行监测对患者肿瘤的诊断具有一定价值。

Objective To explore the expression of CD166 and transcription factor MALAT1 gene in the occurrence and development of non-small cell lung cancer （NSCLC）. Methods 100 cases of patients undergoing lung cancer radical resection with pathological diagnosed NSCLC were chosen as the research object. The selected fresh NSCLC tissues were used as observation group, tissue adjacent to carcinoma as the control group. The CD166 and MALAT1 mRNA expression in both groups were determined by immunohistochemieal and RT-PCR. Results The observation group＇s CD166 positive expression rate was significantly higher than the control group （ P 〈 0. 05 ）. Observation group MALAT1 expression was significantly higher than the control group（P 〈 0.05 ）. The MALAT1 and CD166 expression were related with pathological grade（P 〈 0.05 ）. Conclusion The CD166 and MALAT1 gene transcription factor might have important role in the process of occurrence and development of NSCLC and might have certain value in the diagnosis of NSCLC.