摘要
目的研究IL-8对肺癌细胞增殖和迁移的影响,初步探讨IL-8调控肺癌细胞的分子机制。方法体外培养肺癌NCI-H157细胞,用不同浓度的IL-8刺激肺癌细胞,分别用MTT法检测IL-8对肺癌细胞的增殖作用;划痕损伤实验和Transwell小室两种方法检测肺癌细胞的迁移能力;Western blot检测Rac1和Cdc 42蛋白的表达变化。结果 IL-8促进NCI-H157细胞增殖,但随浓度增加,细胞增殖活性差异无统计学意义;细胞划痕损伤和Transwell实验均表明IL-8可诱导NCI-H157细胞迁移,且具有浓度依赖性;Western blot结果显示,随着IL-8浓度增加,Rac1和Cdc 42的表达水平逐渐升高,以Cdc42变化最为显著。结论 IL-8可促进肺癌细胞增殖和迁移,可能与Rac1和Cdc42表达有关。
Objective To investigate the viability and migration of lung cancer NCI-H147 cells after IL-8 stimulation, and further study the molecular mechanism. Methods The NCI-H157 cells was cultured in vivo, and it was stimulated by different concentrations of IL-8. The cell viability was detected by MTT assays and the migration ability was examined by using the wound-healing and the Transwell assays, respectively. The expression of Rac 1 and Cdc 42 were both detected by Western blot assay. Results The MTF assay showed that the viability of NCI-H147 was increased with different concentrations. Both the Wound-healing and the Transwell assays indicated the migration of NCI-H157 was enhanced dramatically with a dose-dependent manner. The Western blot assays indicated that the expression of Racl and Cdc42 were up-regulated with increasing the concentrations of IL-8, and the change of Cdc42 showed more apparently. Conclusion The cell viability and migration were increased by IL-8, which may be associated with the expression of Racl and Cdc42.
出处
《标记免疫分析与临床》
CAS
2015年第7期682-686,共5页
Labeled Immunoassays and Clinical Medicine