馆藏纸质书画文物上霉菌的分离与鉴定

Isolation and identification of molds from ancient Chinese calligraphy and paintings in museums

霉菌污染会影响馆藏纸质书画的保存和展示。对书画污染菌进行分离及鉴定,有助于提高文物霉变防治工作的针对性,进而提高防治效果。本研究利用传统的纯培养法从遭受霉菌污染的馆藏纸质书画表面分离得到8株霉菌,通过形态特征观察和r DNA转录间隔区序列ITS(Internal Transcribed Spacer)测序分析。结果表明:8株霉菌分属于曲霉属(Aspergillus)、根霉属(Rhizopu)和木霉属(Trichoderma)。研究结果可为馆藏纸质书画文物防霉剂的研发和文物霉菌的综合防治提供靶标和依据。

Contamination by mold is a major potential risk for ancient Chinese calligraphy and paintings on display in museums. The isolation and identification of these microorganisms is helpful for developing a useful approach to control deterioration. In this study, the traditional pure culture -dependent method was performed, and 8 strains of mold were isolated from the paper paintings. In addition to typical mold characteristics, they were identified by ITS sequencing. The results of the morphological and molecular biological investigations show that Aspergillus spp. , Rhi- zopus spp. and Trichoderma spp. are present in these in these specimens.