柯萨奇病毒A16在恒河猴外周血单个核细胞中的增殖研究

Study on proliferation of Coxsackievirus A16-infected peripheral blood mononuclear cells in rhesus monkeys

柯萨奇病毒A16（CA16）感染灵长类动物模型显示该病毒在猴体内可形成明显的病毒血症期，在此基础上，本研究进一步分析病毒血症形成与CA16在外周血单个核细胞（PBMC ）不同组分中增殖的关系。首先用CA16感染恒河猴，检测病毒在PBMC组分CD4＋、CD8＋、CD20＋、CD11c＋、CD16＋和CD14＋细胞中的增殖情况和感染动力学，发现病毒仅在部分CD14＋细胞中有增殖表现。然后通过刺激CD14＋细胞产生一定量的树突细胞（DC），用CA16感染DC ，发现 CA16能在DC中形成具有动力学意义的增殖过程，增殖峰值出现在感染后12～36 h。这些结果可能为CA16感染发病机制提供重要信息。

Previous study suggested that primate animal model infected by Coxsackievirus A 16 （CA16 ） can display a significant viremia phase .This study was conducted for further analyzing the relationship between viremia and viral proliferation in peripheral blood mononuclear cell （PBMC） population .Firstly ,the rhesus monkeys infected with CA16 were detected for the viral loads in CD4＋ ,CD8＋ ,CD20＋ ,CD11c＋ ,CD16 ＋and CD14＋ cells from the PBMCs at different infectious stages ,and the results revealed that CA16 could only propagate in some CD14＋ cells .Secondly ,in the dendritic cells （DCs） derived from CD14＋ cells induced by granulocyte‐macrophage colony stimulating factor （GM‐CSF ） and interleukin 4 （IL‐4 ） , a significant viral proliferation dynamics could be observed with a viral load peak at 12‐36 h post‐infection （hpi） .These findings will likely provide important information on the pathogenesis of CA 16 infection .