全氟化碳对人脐静脉内皮细胞缺氧/复氧损伤的影响

Effects of perfluorocarbon on hypoxia/reoxygenation injury of human umbilical vein endothelial cells

目的:研究全氟化碳(perfluocarbon,PFC)对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)缺氧/复氧损伤模型的保护作用。方法:采用化学方法(连二亚硫酸钠溶液法)制备缺氧/复氧(H/R)损伤模型。实验分组为对照组、10%PFC组、H/R组、PFC+H/R处理组(5%、10%、20%)(以上均为培养液体积比)。CCK-8法测定细胞活力;比色法依次检测培养液中乳酸脱氢酶(1actate dehydrogenase,LDH)外漏量和胞内丙二醛(malondialdehyde,MDA)、一氧化氮(nitric oxide,NO)的含量及超氧化物歧化酶(superoxide dismutase,SOD)的活性。结果:与对照组相比,10%PFC组中各待检指标差异均不明显(P>0.05)。相比于模型组,10%、20%PFC处理组能提高细胞活力A、降低胞内NO含量(P<0.05);5%、10%、20%PFC处理组能显著降低LDH外漏量、胞内MDA含量及提高SOD活性(P<0.01)。结论:PFC对HUVECs缺氧/复氧损伤具有明显的保护作用,其作用机制可能与抑制氧化应激及炎症反应,提高细胞抗氧化的防御能力有关。

Objective：To study the protective effects of perfluorocarbon（PFC）on the injury of human umbilical vein endothelial cells（HUVECs）induced by hypoxia/reoxygenation（H/R）.Method：H/R models were established by sodium hydrosulfite,and the experiment was divided into 6groups：control group,PFC group with PFC at a 10% volume,H/R group,and three PFC＋H/R treatment groups（the volume ratios of culture medium were 5%,10%,20%）.The cell viability was detected by CCK-8 method.The contents of malondialdehyde（MDA）,nitric oxide（NO）and the activities of superoxide dismutase（SOD）in cells,as well as the lactate dehydrogenase（LDH）in the culture medium,were measured by biochemistry approaches.Result：Compared with the control group,every detected index of 10% PFC group had no statistical significance（P〉0.05）.Compared with the model group,10% and 20% PFC treatment groups could increase cell viability and decrease the content of NO（P〈0.05）;Every PFC treatment group could significantly reduce the release of LDH and the contents of MDA,and also increase the activities of SOD（P〈0.01）.Conclusion：PFC has a significant protective effect on hypoxia/reoxygenation injury of HUVECs;and its mechanism may be related to the inhibition of oxidative stress and inflammation so that enhance the ability of cells antioxidant.