络治法对STZ诱导的糖尿病大鼠视网膜NF-κB表达的影响

Influences of collaterals therapy on expression of NF-κB in retina of STZ induced diabetic rats

目的观察络治法对链佐脲菌素(STZ)诱导的糖尿病大鼠视网膜组织中核转录因子κB(NF-κB)表达的影响,探讨络治法对糖尿病视网膜病变微血管损伤的保护作用及作用机制。方法 60只雌性Wistar大鼠随机分成正常组、模型组、羟苯磺酸钙对照药物组、络治组四组。除正常组不做处理外,其它各组均采用STZ腹腔注射制作糖尿病大鼠模型。给药24周后每组大鼠取右眼做眼球壁石蜡切片,行NF-κB免疫组织化学染色,计算机图像定量分析灰度值。左眼取视网膜组织,Western blot法检测视网膜中NF-κB p65蛋白含量。结果免疫组化结果显示络治组视网膜中NF-κB表达明显低于模型组及对照组(P<0.05),灰度值与空白组大体相当(P>0.05)。Western blot结果显示络治组NF-κB p65表达较模型组及对照组显著降低(P<0.05)。结论络治法可明显抑制视网膜组织中NF-κB的活化,甚至接近正常大鼠水平。这可能是络治法对糖尿病大鼠视网膜微血管病变发挥防治作用的重要机理之一。

OBJECTIVE To observe the expression of nuclear factor kappa B （NF-κB） in the retina of streptozotocin （STZ） induced diabetic rats treated with collaterals therapy, and to investigate the protective effects and mechanism of collaterals therapy on diabetic retinopathy microvascular injury. METHODS Sixty female Wistar rats were randomly divided into 4 group including blank group, model group, control group and collaterals therapy group. Except the blank group rats all were made into models of diabetic rats and treated according to the scheme. After 24 weeks each ratˊs right eye was made into parafin section and treated by immunohistochemical staining to detect NF-κB content. While by western blot method detect the NF-κB p65 protein content in left eyeˊs retina was detected. RESULTS Immunohistochemical results showed that the NF-κB expression in the collaterals therapy group was obviously lower than that of model group and control group （P＆lt;0.05）, and was almost the same with the blank group （P＆gt;0.05）. Western blot results showed that the NF-κB p65 expression in collaterals therapy group decreased significantly compared with model group and control group （P＆lt;0.05）. CONCLUSIONS Collaterals therapy could obviously inhibit the expression of NF-κB in retinal tissue, which may be an important mechanism on how collaterals therapy could prevent retinal microvascular lesions.