摘要
目的观察转化生长因子β(TGF-β)抑制剂SB-505124对U87MG细胞放疗敏感性的影响。方法将U87MG细胞分为SB-505124处理组(A组)和对照组(B组),分别接受0、3、6、10和15Gy射线照射后,采用细胞计数试剂盒(CCK-8)检测细胞存活率,免疫荧光技术检测细胞损伤情况。结果 A组和B组射线照射后磷酸化H2AX荧光点数均较照射前增加[(9.02±1.96)个vs.(2.17±0.99)个和(8.75±2.07)个vs.(2.68±0.96)个](P<0.05);但A组与B组射线照射后细胞存活率、荧光点数均无统计学差异(P>0.05)。结论 SB-505124不能增加胶质母细胞瘤对放疗的敏感性。
Objective To investigate the effect of transforming growth factor‐β（TGF‐β） inhibitor SB‐505124 on the sensitivity of glioma cell line U87MG to radiotherapy .Methods U87MG cells were randomly divided into two groups of A （treated with SB‐505124 10 μmol/L for 24 hours） and B（normal control） .After 0 ,3 ,6 ,10 and 15 Gy irradiation ,cell survival and injury were detected by cell counting kit（CCK‐8） and immunofluorescence ,respectively .Results The fluorescent numbers of phosphorylated H2AX after irradiation were more than those before irradiation in groups of A and B [（9.02 ± 1.96） pieces vs .（2.17 ± 0.99） pieces and （8.75 ± 2.07） pieces vs .（2.68 ± 0.96） pieces] （P0 .05） .Conclusion SB‐505124 can not increase the sensitivity of glioma cells to radiotherapy .
出处
《江苏医药》
CAS
2015年第12期1390-1392,共3页
Jiangsu Medical Journal
