格列齐特对小鼠成骨细胞样细胞 MC3T3-E1增殖及分化的影响

Effect of gliclazide on the proliferation and differentiation in mouse osteoblastic-like cells MC3T3-E1

采用 CCK-8和 RT-PCR 技术研究格列齐特对正糖培养的 MC3T3-E1细胞增殖及分化的影响。格列齐特促进 MC3T3-E1细胞增殖和相关功能蛋白 I 型胶原（COL-1）、骨桥蛋白（OPN）和成骨因子Runt 相关基因2（Runx2）基因的表达，在20μmol/ L 时作用最显著，且 COL-1、OPN 与 Runx2基因的表达呈正相关。

[Summary] To investigate the effect of gliclazide on the proliferation and differentiation in MC3T3-E1 cells exposed to normal glucose concentration by applying CCK-8 and RT-PCR. Gliclazide improved the proliferation and stimulated COL-I, OPN and Runx2 mRNA expression in MC3T3-E1 cells, the best concentration of gliclazide was 20μmol/ L, the expression of COL-1 and OPN mRNA had a significant positive correlation with Runx2 binding activity.