运动员有氧和无氧训练后脉冲电流刺激肝区对其血清自由基的影响

Effect of percutaneous stimulation at the hepatic region by impulsive currents on levels of serum free radicals in athletes after aerobic and anaerobic training

目的 通过脉冲电流经皮刺激有氧和无氧训练疲劳运动员肝区,观察体内血清谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、总抗氧化能力活性（T-AOC）及丙二醛（MDA）的变化,探讨运动员有氧和无氧训练经皮刺激肝区对其血清学自由基的影响. 方法 随机抽取某体育学校160名的健康汉族男性运动员,随机分为有氧训练对照组（A组）、无氧训练对照组（B组）、有氧训练刺激组（C组）和无氧训练刺激组（D组）,每组40名,建立训练疲劳模型,刺激组于每天下午体能训练结束后休息20 min,给予肝区中频脉冲的电流刺激.所有运动员于测试前和第1、3、5周日清晨抽取空腹静脉血,检测血清中GSH-Px、SOD、T-AOC、MDA的变化.结果 训练前各检测指标组间比较差异均无统计学意义（P＞0.05）.训练后GSH-Px活力第1周A组＜B组=C组＜D组（P＜0.05）;第3、5周A组=B组＜C组＜D组（P＜0.05）;SOD活力第1周A组＜B组=C组＜D组（P＜0.05）;第3、5周A组=B组＜C组＜D组（P＜0.05）;T-AOC活力第1周A组＜B组＜C组＜D组（P＜0.05）,第3、5周A组=B组＜C组＜D组（P＜0.05）;MDA含量第1周B组＞A组＞C组=D组（P＜0.05）,第3、5周A组=B组＞C组＞D组（P＞0.05）.结论 脉冲电流经皮刺激运动员肝区可以提高血清抗氧化酶的活性,促进机体自由基的消除;对无氧训练疲劳恢复明显优于有氧训练.

Objective To investigate the effect of percutaneous stimulation at the hepatic region by impulsive currents on levels of glutathione peroxidase （GSH-Px）,superoxide dismutase （SOD）,total antioxidant capability （T-AOC） and malondialdehyde （MDA） in athletes after aerobic and anaerobic training.Methods Recruited for the present study were 160 healthy male athletes of Han nationality from a college of sports as candidates for models of training fatigue.They were equally randomized to receive simple aerobic training （Group A）,simple anaerobic training （Group B）,aerobic training plus percutaneous stimulation at the hepatic region by impulsive currents （Group C）,and anaerobic training plus percutaneous stimulation at the hepatic region by impulsive currents （Group D）.The stimulation by impulsive currents of medium frequency was performed 20 minutes after training for 30 minutes every afternoon.Venous blood of 5 ml was collected in each group in fasting condition before the training and on Sundays of the first,third and fifth weeks after the training to measure serum levels of GSH-Px,SOD,T-AOC and MDA.Results There were no significant differences between the 4 groups regarding all the test indexes before the training （P 〉 0.05）.The activity of serum GSH-Px：group A 〈 group B =group C 〈 group D at the first week after the training （P 〈 0.05）;group A =group B 〈 group C 〈 group D at the third and fifth weeks after the training （P 〈 0.05）.The activity of serum SOD：group A 〈 group B =group C 〈 group D at the first week after the training （P 〈 0.05）;group A =group B 〈 group C 〈 group D at the third and fifth weeks after the training （P 〈 0.05）.The activity of T-AOC：group A 〈 group B 〈 group C 〈 group D at the first week after the training （P 〈 0.05）;group A =group B 〈 group C 〈 group D at the third and fifth weeks after the training （P 〈 0.05）.The MDA level：group B 〉 group A 〉 group C =group D at the first week after the training （P 〈 0.05）;group A =group B 〉 group C 〉 group D at the third and fifth weeks after the training （P 〈 0.05）.Conclusions Since percutaneous stimulation at the hepatic region by impulsive currents can enhance activity of serum antioxidant enzymes and reduce levels of free radicals in athletes after aerobic and anaerobic training,it exerts an obvious anti-fatigue effect on athletes.Its effect on anaerobic training is better than on aerobic training.