黄河流域和长江流域棉区绿盲蝽对高效氯氰菊酯和毒死蜱的抗性监测

Resistance to beta-cypermethrin and chlorpyrifos in populations of Apolygus lucorum from the Yellow and Changjiang River Cotton Growing Areas of China

【目的】为了明确绿盲蝽Apolygus lucorum不同地理种群对化学杀虫剂的抗性现状及发展趋势,并为制定有效的化学防治措施及抗性治理策略提供参考,【方法】本文采用玻璃瓶药膜法,连续4年监测了采自黄河流域、长江流域棉区绿盲蝽田间种群对高效氯氰菊酯和毒死蜱的抗性水平及变化动态。【结果】（1）与室内饲养的廊坊敏感品系（Susceptible strain from Langfang,SLF）相比,长江流域棉区江苏南京及安徽滁州种群对2种药剂均未产生明显抗性（〈5倍）,江苏盐城种群对高效氯氰菊酯（3.4~8.5倍）和毒死蜱（3.3~5.8倍）仅有低水平抗性;（2）黄河流域棉区河南安阳、山东滨州及河北沧州种群对高效氯氰菊酯（7.1~22倍）、毒死蜱（5.2~20倍）均产生了中低水平抗性。【结论】2011至2014年间,各地理种群对两种药剂的敏感性在不同年度之间变异幅度小于3倍,抗性发展比较缓慢。黄河流域、长江流域棉区绿盲蝽田间种群对上述2种药剂敏感性的差异可能与当地用药历史和用药量有关。

[Objectives] To investigate insecticide resistance levels in different Apolygus lucorum populations and to thereby improve both the chemical control of this pest and resistance management. [Methods] Samples of A. lucorum populations were collected from the Yellow River Cotton Growing Area（YRCA） and the Changjiang River Cotton Growing Area（CRCA） between 2011 and 2014 and their resistance to beta-cypermethrin and chlorpyrifos measured in a glass-vial bioassay. [Results] Compared with a susceptible laboratory strain（SLF）, populations from Nanjing in Jiangsu Province and Chuzhou in Anhui Province（CRCA） were still susceptible to the two insecticides（ 〈5-fold resistance）, but populations from Yancheng in Jiangsu Province（CRCA） had low levels of resistance to beta-cypermethrin（3.4-8.5 fold resistance） and chlorpyrifos（3.3-5.8 fold resistance）. However, populations collected from Anyang in Henan Province, Binzou in Shandong Province and Cangzhou in Hebei Province（YRCA） had developed low to middle levels of resistance to beta-cypermethrin（7.1- 22 fold） and chlorpyrifos（5.2-20 fold）. [Conclusion] Variation in susceptibility to the 2 insecticides was less than 3 fold between years for each of the 6 monitoring locations. Differences in resistance levels between populations in the YRCA and CRCA may be attributed to the history and the amount of insecticide used locally to control A. lucorum.