结肠癌肝转移机制的研究

Study on mechanism of liver metastasis of colon cancer

目的:研究影响结肠癌肝转移的可能机制。方法 :脾脏注射小鼠结肠癌细胞,构建大鼠结肠癌肝转移模型。实验分成正常组、门静脉分支结扎组、门静脉结扎组和CCl4诱导肝硬化动物模型组,检测各组大鼠肿瘤肝转移发生率、门静脉压力(portal pressure,PP)、血浆丙氨酸转氨酶(alanine aminotransferase,ALT)水平、肝细胞生长因子(hepatocyte growth factor,HGF)和表皮生长因子(epidermal growth factor,EGF)水平。结果 :PP的变化与肿瘤肝转移发生率不完全一致,未发现血浆ALT水平和肝脏转移肿瘤发生之间具有相关性。肝脏HGF和EGF水平与肿瘤肝转移发生率密切相关。结论:影响结肠癌肝转移发生率的因素有很多,除PP外,HGF和EGF所构成的肝脏微环境变化也起重要作用。

Objective To study the possible mechanism of liver metastasis of colon cancer. Methods The liver metastasis model of colon cancer in the rat was induced by injection of murine colon carcinoma cells in spleen. There were normal group, portal branch ligation group, portal vein ligation group and the group of liver cirrhosis induced by CCl4 in this study. The incidence of liver metastasis, portal pressure（PP）, alanine aminotransferase of plasma（ALT）, hepatocyte growth factor（HGF） and epidermal growth factor（EGF） were analyzed. Results Both PP and plasma ALT levels was not related to liver metastasis incidence. The levels of liver HGF and EGF, however, were closely related to the incidence of liver metastasis. Conclusions There are many factors that affect the liver metastases of colon cancer, including HGF and EGF in addition to PP.