结核分枝杆菌Rv3134c蛋白抗原表位的预测

Prediction of epitopes of Mycobacterium tuberculosis Rv3134c protein

目的预测结核分枝杆菌Rv3134c的抗原表位,了解其免疫学特性。方法利用DNAStar软件包中Protean软件对Rv3134c氨基酸序列进行分析,采用包括二级结构、亲水性、抗原性、表面可能性、柔韧性等多参数预测其二级结构及T细胞和B细胞抗原表位,最后BLAST分析其与人类抗原表位的同源性。结果 Rv3134c蛋白具有丰富的二级结构和多处抗原指数较高的区段,含有较多潜在的B细胞抗原表位,可能位于1-7、30-37、65-81、89-100、111-120、138-148、184-195、209-216、233-238位氨基酸残基或其附近,这些区域基本上含有β转角结构,亲水性、表面可能性和柔韧性指数都较高。该蛋白潜在的T细胞抗原表位较少,可能位于62-76、89-97、185-195、203-213、219-231、248-255位氨基酸残基或其附近。BLAST分析结果显示,其与人类抗原表位的同源性很低。结论结核分枝杆菌Rv3134c是一个即含有较多B细胞抗原表位又含有较多T细胞抗原表位的蛋白抗原,实验结果为该蛋白抗原表位的进一步研究与合成肽疫苗奠定了基础。

In this study,we predicted the epitopes of Mycobacterium tuberculosis Rv3134 cprotein and investigated its immunological characteristics.The amino acid sequence of Rv3134 cprotein was input and predicted the epitopes of B cell and T cell using multi-parameters including the secondary structure,hydrophilicity,antigenicity,accessibility,flexibility,charge distribution by Protean software of DNAStar software package.Results showed that the Rv3134 cprotein had rich secondary structure and multiple sections with higher antigenicity.There were a few potential B cell epitopes at 1-7,30-37,65-81,89-100,111-120,138-148,184-195,209-216,and 233-238 amino acid residues or nearby,and theses epitopes had better antigenicity,contained beta angle and irregular coil structure,presented in the surface probability and had larger flexibility.There also were more potential T cell epitopes of the protein containing at 62-76,89-97,185-195,203-213,219-231,248-255,amino acid residues or nearby.There was low homology between Rv3134 cprotein and human protein by BLAST analysis.In conclusion,Mycobacterium tuberculosis Rv3134 cprotein had more B-cell epitopes and T-cell epitopes,which will lay the foundation for its further study and the development of vaccine.