缺血性卒中患者血浆葡萄糖脑苷脂酶和蛋白磷酸酶2A及神经酰胺水平的变化

Alterations of plasma glucocerabrosidase,protein phosphastase 2A and ceramide levels in patients with ischemic stroke

目的分析缺血性卒中患者血浆葡萄糖脑苷脂酶(GBA)、蛋白磷酸酶2A(PP2A)及其降解产物神经酰胺的改变。方法回顾性纳入2013年5月至9月武警后勤学院附属医院神经内科急性缺血性卒中住院患者45例,另收集同期年龄和性别相匹配的45名体检中心的健康者为对照组。采集患者和健康受试者静脉血,抗凝分离血浆。采用蛋白质芯片H50和激光解析电离飞行时间质谱方法测试血浆神经酰胺水平。结果缺血性卒中患者血浆GBA和PP2A水平均明显低于对照组,缺血性卒中组和对照组的GBA水平分别为(2.4±0.8)、(13.1±1.4)U/L,差异有统计学意义(P<0.05),而PP2A水平分别为(6.5±2.8)、(14.5±4.7)U/L(P<0.01)。缺血性卒中患者血浆神经酰胺相对量为1.9±0.7,明显低于对照组的12.2±5.0(P<0.01)。结论缺血性卒中患者血浆GBA和PP2A以及神经酰胺水平均降低,提示卒中患者血液中α-突触核蛋白的异常磷酸化。

Objective To analyze the alterations of plasmaglucocerebrosidase（ GBA）,protein phosphatase 2 A（ PP2 A） and its degradation product ceramide in patients with ischemic stroke.Methods A total of 45 inpatients with ischemic stroke at the Department of Neurology,the Affiliated Hospital of Logistics College of the CAPF were enrolled from May to September 2013,and 45 age- and sex-matched healthy subjects at the Physical Examination Center collected at the same time period were used as a control group. Blood samples of the patients and healthy subjects were obtained,anticoagulated,and the plasma was separated. H50 protein chip and laser matrix-assisted laser desorption / ionization top of fly mass spectrometry were used to test the levels of plasma ceramide. Results The plasma GBA and PP2 A activities in patients with ischemic stroke were significantly lower than those of the control group; the GBA activities of the ischemic stroke group and the control group were 2. 4 ± 0. 8 and 3. 1 ± 1. 4 U / L respectively. There was significant difference（ P 0. 05）; the PP2 A activities of the two groups were 6. 5 ±2. 8 and 14. 5 ± 4. 7 U / L respectively（ P 0. 01）. The relative level of the plasma ceramide in patients with ischemic stroke was 1. 9 ± 0. 7,and it was significantly lower than 12. 2 ± 5. 0 of the control group（ P 0. 01）. Conclusion The decreased levels of plasma GBA and PP2 A activities as well as the ceramide in patients with ischemic stroke suggested that the abnormal phosphorylation of synuclein in the blood of patients with stroke.