Eales病患者视网膜光凝治疗前后血浆一氧化氮及一氧化氮合酶活性的变化

Plasma nitric oxide level and nitric oxide synthase activity in patients with Eales disease before and after retinal photocoagulation

目的观察和分析Eales病患者视网膜光凝治疗前后血浆一氧化氮(NO)及一氧化氮合酶(NOS)活性的变化。方法选取80例(131只眼)Eales病患者作为观察组,将其中接受视网膜光凝治疗的44例患者(60只眼)作为激光治疗组,将其中未接受视网膜光凝治疗的36例患者(71只眼)作为非激光治疗组,选取40例排除眼病的健康人作为对照组。对所有研究对象治疗前及激光治疗组患者术后1、3、6个月的血浆NO水平及血浆总NOS(t NOS)、结构型NOS(c NOS)和诱导型NOS(i NOS)水平进行检测和比较,并对激光治疗组患者的治疗有效率和视力恢复情况进行评价。结果观察组患者的血浆NO水平、t NOS水平、c NOS水平均显著低于对照组(t=6.128、3.465、4.053,P<0.05),激光治疗组与非激光治疗组患者治疗前的血浆NO水平、t NOS水平、i NOS水平、c NOS水平的差异均无统计学意义(t=0.625、0.511、0.426、0.637,P<0.05);经视网膜光凝治疗后,激光治疗组中视力稳定或提高的患眼数占全部患眼的90%,疗效评价有效的患眼数占全部患眼的88.3%,而患者在治疗前,术后1、3、6个月各时点的血浆NO水平、t NOS水平、i NOS水平、c NOS水平的差异均无统计学意义(F=0.728、0.635、0.608、0.942,P<0.05)。结论 Eales病患者表现为血浆NO、NOS水平显著下降,但这一变化的程度与其视网膜病变程度无关,视网膜光凝治疗能够有效缓解患者的视网膜病变、恢复患者的视力,但对于纠正上述引发Eales病的病理机制无显著作用。

Objective To observe and analyze the changes of plasma nitric oxide （NO）and nitric oxide synthase （NOS）levels in patients with Eales disease before and after retinal photocoagulation. Methods Totally 80 patients with Eales disease （131 eyes）were enrolled. Forty four patients （60 eyes）were treated with retinal photocoagulation （laser group）and 36 patients （71 eyes）received standard non-laser treatment （non-laser group）. Additional 40 healthy people were selected as the control group. The plasma levels of NO and total NOS （tNOS）,structural NOS （cNOS）and inducible NOS （iNOS）were measured before and after treatment at 1 month,3 months,6 months. Visual acuity and the efficiency of laser treatment were evaluated. Results The plasma NO level,tNOS level and cNOS level of the patients received laser treatment were significantly lower than those of control group （t = 6. 128,3. 465,4. 053,P 〈 0. 05）,but not significantly different from those of the patients received non-laser treatment （t = 0. 625,0. 511 and 0. 426,0. 637,P 〉 0. 05）. After retinal photocoagulation,improved or stable visual acuity was found in 90% of the patients treated with laser,and the over-all effectiveness rate was 88. 3% . However,plasma NO,tNOS,iNOS and cNOS levels did not change significantly in these patients at each time point before and after the retinal coagulation （F = 0. 728,0. 635,0. 608,0. 942,P 〉 0. 05）. Conclusion The patients with Eales disease show significant decreases in plasma NO and NOS levels,but the extents of the changes are independent of the degree of the patients’retinopathy. The retinal photocoagulation treatment can effective-ly alleviate the patients’retinopathy,restore patient＆#39;s visual acuity,but puts no significant effects on correcting the patho-logical mechanism of Eales disease.