A549和SK-OV-3细胞株中CD44v6、整合素αvβ3、EGFR及E-钙黏素的表达差异与远处转移关系

Difference in expression of CD44v6,integrin αvβ3,EGFR and E-cadherin between human lung cancer A549 and ovarian cancer SK-OV-3 cell lines

目的探讨肺癌A549细胞株和卵巢癌SK-OV-3细胞株中CD44v6、整合素αvβ3、EGFR及E-钙黏素的表达水平差异及其与肺癌和卵巢癌远处转移的关系。方法利用流式细胞仪分组检测A549细胞株和SK-OV-3细胞株中CD44v6、整合素αvβ3、EGFR及E-钙黏素的表达水平。结果 A549细胞株中CD44v6、整合素αvβ3、EGFR和E-钙黏素的表达阳性率均高于SK-OV-3细胞株[(93.48±2.97)%、(95.42±2.26)%、(94.30±3.43)%、(94.52±1.59)%vs(82.73±4.28)%、(83.60±1.26)%、(88.52±0.88)%、(86.96±3.07)%,均P<0.05]。结论促转移分子CD44v6、整合素αvβ3、EGFR以及抑制转移分子E-钙黏素在A549细胞株的表达均高于SK-OV-3细胞株,这可能是肺癌比卵巢癌更容易发生远处转移特别是脑转移的部分分子机制。

Objective To investigate the expression of CD44v6,integrin αvβ3,EGFR and E- cadherin in human lung cancer A549 and ovarian cancer SK- OV- 3 cell lines and their biological significance. Methods Expression levels of CD44v6,integrin αvβ3,EGFR and E- cadherin in A549 and SK- OV- 3 cell lines were detected by flow cytometry.Results The positive rates of CD44v6,integrin αvβ3,EGFR and E- cadherin in A549 cells were significantly higher than those in SK- OV- 3 cells [（ 93. 48 ± 2. 97） % vs（ 82. 73 ± 4. 28） %,（ 95. 42 ± 2. 26） % vs（ 83. 60 ± 1. 26） %,（ 94. 30± 3. 43） % vs（ 88. 52 ± 0. 88） % and（ 94. 52 ± 1. 59） % vs（ 86. 96 ± 3. 07） %,respectively,all P 0. 05]. Conclusions The positive rates of CD44v6,integrin αvβ3,EGFR and E- cadherin in A549 cells are all higher than those in SK-OV-3cells. The integrated effects of these molecules may be associated with more distant metastases in lung cancer than in ovarian cancer.