EBER、PTEN和VEGF在血管免疫母T细胞淋巴瘤中的表达及其临床病理学意义

Expression and Clinical Pathological Significance of EBER,PTEN and VEGF in Angioimmunoblastic T-Cell Lymphoma

目的:探讨血管免疫母细胞T细胞淋巴瘤(angioimmunoblastic T-cell lymphoma,AITL)组织中EB病毒(Epstein-Barr virus,EBV)、PTEN和VEGF三者表达间的关系,并分析三者与患者临床病理学参数间的关系。方法:采用原位杂交法检测21例AITL组织中EBV编码的小RNA(EBER)的表达情况,免疫组织化学En Vision两步法检测21例AITL和20例淋巴结反应性增生组织中PTEN和VEGF的表达情况,分析三者表达间的相关性及其与患者临床病理学参数间的关系。结果:21例血管免疫母细胞T细胞淋巴瘤中EBER表达阳性率为61.9%;PTEN和VEGF在AITL和淋巴结反应性增生组织中的表达差异均具有显著性(P<0.05);EBER与PTEN表达呈负相关(P<0.05)。血管免疫母细胞T细胞淋巴瘤中,男性患者和进展期组EBER阳性表达率分别为80%和78.6%,明显高于女性患者和非进展期组患者(P<0.05);在伴随B症状和进展期组PTEN阳性表达率分别为31.3%和21.4%,明显低于无B症状和非进展期组患者(P<0.05)。生存分析显示,PTEN表达与患者总生存率呈负相关(P<0.05)。结论:EBV感染和PTEN低表达可能预示血管免疫母细胞T细胞淋巴瘤恶化进展。EBV是否通过下调PTEN表达参与血管免疫母T细胞淋巴瘤的发生尚不清楚,需进一步研究探讨。

Objective： To investigate the expression and clinical pathological significance of EB virus （ Epstein-Barr virus, EBV）, PTEN and VEGF in angioimmunoblastic T -cell lymphoma （AITL）. Methods： The EBV -encoded small RNA （EBER） expression in 21 cases of AITL was detected by in situ hybridization. The expressions of PTEN and VEGF were detected in 21 cases of AITL and 20 cases of lymph node reactive hyperplasia by immunohistochemical EnVision two-steps method. The expression and clinicopathological significance of EBV, PTEN and VEGF in AITL were analyzed. Results： The positive expression rate of EBER in 21 cases of AITL was 61.9% ; the expressions of PTEN and VEGF in AITL and lymph node reactive hyperplasia were significantly different（P 〈 0.05 ）. The expressions of EBER and PTEN negatively correlated （ P 〈 0.05 ）. The EBER positive expression rates of male patients in AITL group and the progressed group was 80% and 78.6% respectively, which were significantly higher than that in female patients and patients in nonadvanced group （ P 〈 0.05 ） ; the PTEN expression rates in the AITL group accompanying B symptoms and progressed group were 31.3% and 21.4%, respectively, which were significantly lower than those in patients without B symptoms and non-progressed group （ P 〈 0.05 ）. Survival analysis showed that the PTEN expression negatively correlated with the overall survival rate of patients （ P 〈 0. 05 ）. Conclusion： EBV infection and low expression of PTEN may indicate the deterioration of angioimmunoblastic T -cell lymphoma. Whether the EBV involved in the ocurring of T-cell angioimmunoblastic lymphoma by down-regulating PTEN expression is unclear, further research is needed.