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萝卜硫素对分化型甲状腺癌细胞NIS基因表达及摄碘能力的影响 被引量：3

Effect of sulforaphane on sodium/iodide symporter gene expression and radioidide uptake of differentiated thyroid cancer cell line
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摘要 目的:研究萝卜硫素对分化型甲状腺癌细胞NIS基因表达及摄碘能力的影响。方法:选择乳头状甲状腺癌细胞系K1及滤泡状甲状腺癌细胞系FTC133进行体外培养,给予不同浓度萝卜硫素处理24h,通过反转录实时荧光定量聚合酶链反应(RT-qPCR)检测钠/碘同向转运体(NIS)mRNA的表达;通过γ计数仪检测甲状腺癌细胞摄碘能力。结果:反转录实时荧光定量聚合酶链反应(RT-qPCR)检测结果表明,萝卜硫素可以上调NIS基因的表达,并呈浓度依赖性;γ计数仪检测结果表明,萝卜硫素可以提高分化型甲状腺癌的摄碘能力。结论:萝卜硫素为放射性碘治疗分化型甲状腺癌提供了新的思路。 Objective：To study the effect of Sulforaphane on sodium/iodide symporter gene expression and radioidide uptake of differentiated thyroid cancer cell line .Method：the thyroid cancer cell line K1 and FTC133 were treated with different concentrations of sulforaphane for 24h ,the expression level of NIS mRNA of K1 and FTC133 were determined by RT‐qPCR and their radioiodide concentration activity were measured by γ‐counter .Re‐sults ：Sulforaphane can up‐regulate the expression of NIS in a dose‐dependent manner and also increase the ability of radioiodide concentration of differentiated thyroid cancer line .Conclusion：Sulforaphane provides a new idea for radio‐active iodine treatment of differentiated thyroid cancer .
作者 王丽萍
机构地区 西安市中心医院内分泌科
出处 《陕西医学杂志》 CAS 2015年第7期779-780,784,共3页 Shaanxi Medical Journal
关键词 甲状腺肿瘤/治疗 硫氰酸类 糖蛋白类 碘放射性同位素 基因表达 Thyroid neoplasms/therapy Thiocyanates Glycophorins Iodine radioisotopes Gene expression
分类号 R392.3 [医药卫生—免疫学]
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参考文献6

  • 1Dohán O1,De la Vieja A,Paroder V,et al.The sodium/iodide Symporter(NIS):Characterization,regulation,and medical significance[J].Endocr Rev,2003,24(1):48-77.
  • 2余永利,罗全勇,陈立波,罗琼,丁颖,陆汉魁,朱瑞森,马寄晓.分化型甲状腺癌术后~131Ⅰ治疗生存率分析[J].中华核医学杂志,2006,26(5):261-263. 被引量：21
  • 3Smit J W,Shroder-Van D E J,Karperien M,et al.Reestablishment of in vitro and in vivo iodide uptake by transfection of the human sodium iodide symporter(hNIS)in a hNIS defectivehuman thyroid carcinoma cell line[J].Thyroid,2000,10(11):939-943.
  • 4Zarnegar R,Brunaud L,Kananchi H,et al.Increasing the effectiveness of radioactive iodine therapy in the treatment of thyroid cancer using Trichostatin A,a histone deacetylase inhibitor[J].Surgery,2002,132(6):984-990.
  • 5Myzak MC,Hardin K,Wang R,et al.Sulforaphane inhibits histone deacetylase activity in BPH-1,LnCaP and PC-3prostate epithelial cells[J].Carcinogenesis,2006,27:811-819.
  • 6Pledgie-Tracy A,Sobolewski MD,Davidson NE.Sulforaphane induces cell type-specific apoptosisin human breast cancer cell lines[J].Mol Cancer Ther,2007,6:1013-1021.

二级参考文献8

  • 1Kouvaraki MA,Shapiro SE,Lee JE,et al.Surgical management of thyroid carcinoma.J Natl Compr Canc Netw,2005,3:458-466.
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共引文献20

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引证文献3

二级引证文献12

陕西医学杂志
2015年 第7期

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