人血高铁血红蛋白浓度快速检测卡的研制

Invention of a rapid detection card for blood methemoglobin concentration in human

目的：明确亚硝酸钠溶液与高铁血红蛋白生成率间的量效关系,研制人血高铁血红蛋白浓度检测卡,为法医检案、侦查及临床医务工作者提供一种快速、安全且经济的检测方法。方法：建立亚硝酸钠溶液与高铁血红蛋白生成率间的量效方程,获取制备10%~80%（间隔10%）高铁血红蛋白血样所需的亚硝酸钠溶液浓度。将健康人与10%~80%高铁血红蛋白血样分别滴于血样采集卡,扫描获取红绿蓝（red,green,blue,RGB）值,生成色块后,制成检测卡。结果：不同浓度的亚硝酸钠溶液与高铁血红蛋白生成率呈直线正相关。随着高铁血红蛋白血样浓度增高,其颜色在血样采集卡上逐渐变深。结论：该检测卡为人血高铁血红蛋白的检测提供了快速、安全且经济的方法,值得推广。

Objective：To investigate the relation between nitrite solutions with different concentrations and methemoglobin formation rates,to invent a rapid,safe and economical detection card for blood methemoglobin concentration in human and to provide references for forensic science and clinical work. Methods：According to the dose-effect equation between nitrite solutions with different concentrations and methemoglobin formation rate,nitrite solutions with different concentrations were obtained for generating methemoglobin blood samples with different concentrations（10%-80%）. Then,blood samples from healthy donors and methemoglobin blood samples with different concentrations were dropped on the blood collection cards. The values of red,green,blue（RGB）were acquired,and different methemoglobin color-pieces were generated. Finally,the rapid detection card was successfully fabricated. Results：Nitrite solution with different concentrations and methemoglobin formation rates was in linear correlation.With the increasing of concentration of methemoglobin in the blood sample,the color of the detection card became more and more dark. Conclusion：This detection card is rapid,safe and economical for methemoglobin concentration detection. Therefore,it might be widely applied in the future.