意向运动疗法对大鼠脑缺血后Nogo-A和Rho激酶表达的影响

Effect of willed movement therapy on the expression of Nogo-A and Rho-associated kinase in rats of cerebral ischemia

目的 探讨意向运动疗法对大鼠脑缺血周围区Nogo-A和Rho激酶表达的影响.方法 采用线栓法建立大鼠大脑中动脉栓塞（MCAO）模型,再灌注24 h后根据Longa神经功能缺损评分选择模型.将54只造模成功的雄性SD大鼠按照随机数字表法分为MCAO模型组（18只）、环境改变组（18只）、意向运动组（18只）,每组再细分为3d、7d、15d3个亚组,每组6只.MCAO组大鼠饲养于常规饲养箱,自由饮食、进水,环境改变组及意向运动组大鼠均饲养于自制饲养箱中,其中意向运动组大鼠所处饲养箱的饮水瓶及饲料均位于箱顶.大鼠处死前再进行Longa评分,采用免疫组织化学染色法观察大鼠缺血周围区脑组织Nogo-A和Rho激酶的表达水平.结果 再灌注15d,意向运动组大鼠神经功能缺损评分低于MCAO模型组及环境改变组（P＜0.05）.再灌注7d及15d,意向运动组大鼠Nogo-A表达水平[（28.92±2.17）个/高倍镜视野和（24.38±2.29）个/高倍镜视野]及Rho激酶[（40.03±2.14）个/高倍镜视野和（38.08±2.07）个/高倍镜视野]表达水平均低于MCAO模型组及环境改变组（P＜0.05）.MCAO模型组与环境改变组所有时间点Nogo-A及Rho激酶表达水平之间比较,差异均无统计学意义（P＞0.05）.结论 意向运动疗法可改善MCAO大鼠的神经功能缺损症状,其机制可能与抑制缺血周围区脑组织Nogo-A和Rho激酶的表达水平有关.

Objective To determine the effect of willed movement on the expression of Nogo-A and Rho-associated kinase （ROCK） in adult rats with cerebral ischemia.Methods Cerebral ischemia/reperfusion injury was established by middle cerebral artery occlusion （MCAO） for 2 h,followed by a 24 h reperfusion in 54 adult rats and the degree of their neurological deficit was evaluated using Longa scale.They were then divided randomly into 3 groups,namely the MCAO group,the environmental modification （EM） group,and the willed movement （WM） group.The rats of MCAO group were raised in a regular breeding box,where they could get food and water freely.Meanwhile,those of the other two groups were raised in a homemade box.For the WM group,the water bottle and food were located on the roof of the homemade box.In each group,six rats were killed on day 3,7 and 15 after reperfusion and their neurological deficits were evaluated respectively.Immunohistochemistry assay was employed to examine the expression of Nogo-A and ROCK in the brain tissue around the ischemic foci.Results The rats of the WM group showed lessened neurological deficits on day 15 compared with the model and EM group.Their expression of Nogo-A decreases from（28.92 ± 2.17）/hpf on day 7 to （24.38 ± 2.29）/hpf on day 15 and that of ROCK did from （40.03 ± 2.14）/hpf to （38.08 ± 2.07） / hpf,lower than those of the model and EM group.However,no significant differences were found in the expression of Nogo-A and ROCK between the model group and EM group at any time points.Conclusion Willed movement could promote the functional recovery of neurological deficits in rats with ischemia after reperfusion,which is probably in relation to restrained expression of Nogo-A and Rho-associated in the tissue around the brain ischemic foci.