肺腺癌患者突触小体相关蛋白家族的表达研究

Expression of synaptosomal-associated protein in non-small cell lung cancer

目的大量研究表明,突触小体相关蛋白(synaptosomal-associated protein,SNAP)与多种肿瘤的发生发展密切相关。文中旨在筛选并验证在肺腺癌中特异性表达的突触小体相关蛋白47(synaptosomal-associated protein 47,SNAP47)并分析其与临床指标间的关系。方法应用表达谱基因芯片和肿瘤基因组图谱(the cancer genome atlas,TCGA)公共数据库筛选出特异性高表达于肺癌中的SNAP47,并选取江苏省肿瘤医院有完整临床资料的52例肺腺癌患者,对其癌组织及对应的癌旁肺组织应用实时定量逆转录聚合酶链反应检测SNAP47 mRNA的表达情况,并分析其与临床指标间的关系。结果 52例肺腺癌组织中,41例(78.9%)SNAP47 mRNA表达水平明显高于对应的癌旁肺组织,差异有统计学意义(P<0.05),且Ⅱ、Ⅲ期患者SNAP47 mRNA的表达水平(6.558±4.730)显著高于I期患者(2.718±2.370),N1+N2组患者SNAP47 mRNA的表达水平(6.609±4.942)也明显高于N0组患者(3.360±2.987),差异均有统计学意义(P<0.05)。结论 SNAP47 mRNA特异性高表达可能参与了肺腺癌的浸润及淋巴结转移,是潜在的肺癌诊疗靶点。

Objective Numerous studies had shown that synaptic-associated proteins（ SNAPs） were closely related to the occurrence and development of tumors. The aim of this study was to investigate the expression of synaptosomal-associated protein 47（ SNAP47） and its correlation with the clinicopathological features in non-small cell lung cancer（ NSCLC）. Methods The expressions of SNAP family（ SNAP23,SNAP25,SNAP29 and SNAP47） were extracted and analyzed through the gene expression microarray and the cancer genome atlas（ TCGA） data-base. SNAP47 mRNA expression in 52 cases of lung adenocarcinoma and their corresponding normal tissues were detected by quantitative real-time PCR（ qRTPCR）. Results Among 52 cases of lung adenocarcinoma,SNAP47 mRNA expression levels of 41 cases（ 78. 9%） were significantly higher than the adjacent lung tissue（ P〈0. 05）. The mRNA level of SNAP47 was associated with lymph node invasion and advanced clinical pathological stage. The mRNA levels of SNAP47 of patients in Ⅱ/Ⅲ stage were significantly higher than those of Ⅰ stage patients（ 6. 558 ±4. 730 vs 2. 718 ± 2. 370,P〈0. 05）. The mRNA levels of N1 ＋ N2 were higher than those of N0（ 6. 609 ± 4. 942 vs 3. 360 ± 2. 987,P〈0. 05）. Conclusion The high specificity of SNAP47 expression in lung cancer tissues might be associated with the invasion and lymph node metastasis of NSCLC,which is the potential therapeutic target of lung cancer.