荧光共振能量转移技术在肿瘤研究中的应用进展

Application of fluorescence resonance energy transfer technology in cancer research

近年来,肿瘤基因组学、靶向治疗等方面的研究进展显著扩大了蛋白质-蛋白质相互作用在肿瘤研究中的应用。荧光共振能量转移(fluorescence resonance energy transfer,FRET)是一种非辐射的能量转移,可以定时、定量、定位、动态地观察活细胞内蛋白质与蛋白质之间的相互作用。随着荧光蛋白的发现及基于FRET原理设计的生物探针的发展,FRET已成为观察活细胞内生物大分子之间相互作用的重要方法。文中就目前FRET技术在肿瘤基础研究、早期诊断、预后评价及药物研发等领域中的应用进行综述。

In recent years,the emergence and progress of cancer genomics and targeted therapies have remarkably expanded the application fields of protein-protein interaction in cancer research. Fluorescence resonance energy transfer（ FRET） is a kind of nonradiation energy transfer technique,which can timing,quantitative,positioning,and dynamically monitor the interaction between protein and protein in living cells. With the discovery of novel fluorescent proteins and the development of FRET-based biosensors,FRET has became an important method for visualizing spatial and temporal dynamics of interactions among biological macromolecules in native environments. This review summarises the recent studies and technological advances that have enhanced the use of FRET technology in cancer basic research,early diagnosis,prognosis evaluation,drug development and other areas.